Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5615679..5616321 Replicon chromosome
Accession NZ_CP083156
Organism Bacillus thuringiensis strain ABTS-1857

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z20_RS29385 Protein ID WP_000635965.1
Coordinates 5615971..5616321 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z20_RS29380 Protein ID WP_000004570.1
Coordinates 5615679..5615966 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z20_RS29355 (K8Z20_29065) 5610996..5611958 + 963 WP_000961168.1 UV DNA damage repair endonuclease UvsE -
K8Z20_RS29360 (K8Z20_29070) 5611951..5612523 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
K8Z20_RS29365 (K8Z20_29075) 5612617..5612976 + 360 WP_000583417.1 holo-ACP synthase -
K8Z20_RS29370 (K8Z20_29080) 5613133..5614083 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
K8Z20_RS29375 (K8Z20_29085) 5614201..5615370 + 1170 WP_000390616.1 alanine racemase -
K8Z20_RS29380 (K8Z20_29090) 5615679..5615966 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z20_RS29385 (K8Z20_29095) 5615971..5616321 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z20_RS29390 (K8Z20_29100) 5616389..5618557 + 2169 WP_000426236.1 Tex family protein -
K8Z20_RS29395 (K8Z20_29105) 5618616..5618732 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K8Z20_RS29400 (K8Z20_29110) 5618928..5619386 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216168 WP_000635965.1 NZ_CP083156:5615971-5616321 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216168 NZ_CP112980:3502001-3502300 [Enterobacter hormaechei subsp. xiangfangensis]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCTATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATACTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216168 WP_000004570.1 NZ_CP083156:5615679-5615966 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216168 NZ_CP112980:3502311-3502658 [Enterobacter hormaechei subsp. xiangfangensis]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCGGACGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References