Detailed information of TA system TA216167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2121053..2122031
• Replicon: chromosome
• Accession: NZ_CP083156
• Organism: Bacillus thuringiensis strain ABTS-1857

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: K8Z20_RS10800
• Protein ID: WP_000624977.1
• Coordinates: 2121053..2121790 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: K8Z20_RS10805
• Protein ID: WP_000237818.1
• Coordinates: 2121903..2122031 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z20_RS10780 (K8Z20_10720)	2116696..2117478	+	783	WP_000381800.1	class I SAM-dependent methyltransferase	-
K8Z20_RS10785 (K8Z20_10725)	2117636..2119321	-	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
K8Z20_RS10790 (K8Z20_10730)	2119429..2119911	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
K8Z20_RS10795 (K8Z20_10735)	2120078..2120815	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
K8Z20_RS10800 (K8Z20_10740)	2121053..2121790	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K8Z20_RS10805 (K8Z20_10745)	2121903..2122031	+	129	WP_000237818.1	hypothetical protein	Antitoxin
K8Z20_RS10810 (K8Z20_10750)	2122104..2122280	+	177	WP_000852629.1	stage II sporulation protein SB	-
K8Z20_RS10815 (K8Z20_10755)	2122299..2122688	-	390	WP_000713866.1	YxeA family protein	-
K8Z20_RS10820 (K8Z20_10760)	2122900..2124369	+	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
K8Z20_RS10825 (K8Z20_10765)	2124615..2125424	+	810	WP_000678509.1	papain-like cysteine protease family protein	-
K8Z20_RS10830 (K8Z20_10770)	2125450..2126052	+	603	WP_000517260.1	hypothetical protein	-
K8Z20_RS10835 (K8Z20_10775)	2126292..2126544	-	253	Protein_2116	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T216167 WP_000624977.1 NZ_CP083156:2121053-2121790 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T216167 NZ_CP112980:c2680635-2680532 [Enterobacter hormaechei subsp. xiangfangensis]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT216167 WP_000237818.1 NZ_CP083156:2121903-2122031 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT216167 NZ_CP112980:2680527-2680728 [Enterobacter hormaechei subsp. xiangfangensis]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5