Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5665379..5666021 Replicon chromosome
Accession NZ_CP083144
Organism Bacillus thuringiensis strain GC-91

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z38_RS29520 Protein ID WP_000635965.1
Coordinates 5665671..5666021 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z38_RS29515 Protein ID WP_000004570.1
Coordinates 5665379..5665666 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z38_RS29490 (K8Z38_29455) 5660696..5661658 + 963 WP_000961168.1 UV DNA damage repair endonuclease UvsE -
K8Z38_RS29495 (K8Z38_29460) 5661651..5662223 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
K8Z38_RS29500 (K8Z38_29465) 5662317..5662676 + 360 WP_000583417.1 holo-ACP synthase -
K8Z38_RS29505 (K8Z38_29470) 5662833..5663783 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
K8Z38_RS29510 (K8Z38_29475) 5663901..5665070 + 1170 WP_000390616.1 alanine racemase -
K8Z38_RS29515 (K8Z38_29480) 5665379..5665666 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z38_RS29520 (K8Z38_29485) 5665671..5666021 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z38_RS29525 (K8Z38_29490) 5666089..5668257 + 2169 WP_000426236.1 Tex family protein -
K8Z38_RS29530 (K8Z38_29495) 5668316..5668432 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K8Z38_RS29535 (K8Z38_29500) 5668628..5669086 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216166 WP_000635965.1 NZ_CP083144:5665671-5666021 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216166 NZ_CP112980:c2280411-2279926 [Enterobacter hormaechei subsp. xiangfangensis]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACCTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCAGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216166 WP_000004570.1 NZ_CP083144:5665379-5665666 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216166 NZ_CP112980:c2280665-2280399 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCGGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References