Detailed information of TA system TA216165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2167493..2168471
• Replicon: chromosome
• Accession: NZ_CP083144
• Organism: Bacillus thuringiensis strain GC-91

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: K8Z38_RS11130
• Protein ID: WP_000624977.1
• Coordinates: 2167493..2168230 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: K8Z38_RS11135
• Protein ID: WP_000237818.1
• Coordinates: 2168343..2168471 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z38_RS11110 (K8Z38_11065)	2163136..2163918	+	783	WP_000381800.1	class I SAM-dependent methyltransferase	-
K8Z38_RS11115 (K8Z38_11070)	2164076..2165761	-	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
K8Z38_RS11120 (K8Z38_11075)	2165869..2166351	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
K8Z38_RS11125 (K8Z38_11080)	2166518..2167255	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
K8Z38_RS11130 (K8Z38_11085)	2167493..2168230	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K8Z38_RS11135 (K8Z38_11090)	2168343..2168471	+	129	WP_000237818.1	hypothetical protein	Antitoxin
K8Z38_RS11140 (K8Z38_11095)	2168544..2168720	+	177	WP_000852629.1	stage II sporulation protein SB	-
K8Z38_RS11145 (K8Z38_11100)	2168739..2169128	-	390	WP_000713866.1	YxeA family protein	-
K8Z38_RS11150 (K8Z38_11105)	2169340..2170809	+	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
K8Z38_RS11155 (K8Z38_11110)	2171055..2171864	+	810	WP_000678509.1	papain-like cysteine protease family protein	-
K8Z38_RS11160 (K8Z38_11115)	2171890..2172492	+	603	WP_000517260.1	hypothetical protein	-
K8Z38_RS11165 (K8Z38_11120)	2172732..2172984	-	253	Protein_2178	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T216165 WP_000624977.1 NZ_CP083144:2167493-2168230 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T216165 NZ_CP112980:c1091733-1091515 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT216165 WP_000237818.1 NZ_CP083144:2168343-2168471 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT216165 NZ_CP112980:c1092135-1091761 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5