Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3191800..3192442 Replicon chromosome
Accession NZ_CP083134
Organism Bacillus thuringiensis strain BMP144

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z33_RS16145 Protein ID WP_000635965.1
Coordinates 3191800..3192150 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z33_RS16150 Protein ID WP_000004570.1
Coordinates 3192155..3192442 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z33_RS16130 (K8Z33_16080) 3188736..3189194 - 459 WP_000343552.1 SprT family protein -
K8Z33_RS16135 (K8Z33_16085) 3189390..3189506 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
K8Z33_RS16140 (K8Z33_16090) 3189564..3191732 - 2169 WP_000426211.1 Tex family protein -
K8Z33_RS16145 (K8Z33_16095) 3191800..3192150 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z33_RS16150 (K8Z33_16100) 3192155..3192442 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z33_RS16155 (K8Z33_16105) 3192751..3193920 - 1170 WP_000390594.1 alanine racemase -
K8Z33_RS16160 (K8Z33_16110) 3194038..3194988 - 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
K8Z33_RS16165 (K8Z33_16115) 3195145..3195504 - 360 WP_000583420.1 holo-ACP synthase -
K8Z33_RS16170 (K8Z33_16120) 3195598..3196170 + 573 WP_000908528.1 rhomboid family intramembrane serine protease -
K8Z33_RS16175 (K8Z33_16125) 3196163..3197125 - 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216164 WP_000635965.1 NZ_CP083134:c3192150-3191800 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216164 NZ_CP112980:c301279-300977 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216164 WP_000004570.1 NZ_CP083134:c3192442-3192155 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216164 NZ_CP112980:c300970-300683 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References