Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4774948..4775590 Replicon chromosome
Accession NZ_CP083129
Organism Bacillus thuringiensis strain NB-176

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z23_RS24580 Protein ID WP_000635965.1
Coordinates 4774948..4775298 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z23_RS24585 Protein ID WP_000004570.1
Coordinates 4775303..4775590 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z23_RS24565 (K8Z23_24525) 4771884..4772342 - 459 WP_000343553.1 SprT family protein -
K8Z23_RS24570 (K8Z23_24530) 4772538..4772654 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
K8Z23_RS24575 (K8Z23_24535) 4772712..4774880 - 2169 WP_016078972.1 Tex family protein -
K8Z23_RS24580 (K8Z23_24540) 4774948..4775298 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z23_RS24585 (K8Z23_24545) 4775303..4775590 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z23_RS24590 (K8Z23_24550) 4775899..4777068 - 1170 WP_000390595.1 alanine racemase -
K8Z23_RS24595 (K8Z23_24555) 4777186..4778136 - 951 WP_003306148.1 outer membrane lipoprotein carrier protein LolA -
K8Z23_RS24600 (K8Z23_24560) 4778293..4778652 - 360 WP_000583417.1 holo-ACP synthase -
K8Z23_RS24605 (K8Z23_24565) 4778746..4779318 + 573 WP_016078971.1 rhomboid family intramembrane serine protease -
K8Z23_RS24610 (K8Z23_24570) 4779311..4780273 - 963 WP_016078970.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216162 WP_000635965.1 NZ_CP083129:c4775298-4774948 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216162 NZ_CP112978:2482063-2482464 [Leptospira borgpetersenii]
ATGACTCAATACTTATTAGATACGAATATTTGCATTTATATAATAAACCAAAAGCCGAGATCTGTTTATAAAAAATTTAA
AAAGATCAAGTTAGAAAACATATTTATTTCATCAATTACGGAATTTGAATTAAAGTATGGTGTTCAAAAAAGTCTTCATT
TCGAAAGGAACTTAAAAATTTTAGAAGAGTTTTTAAGTTATTTGAATATTCTTTCATTTGTTAGTGAAGACGCAAATAAA
GCTGCAAAAATTCGAGTAGAATTAGACAAAGTAGGGAAACCAATCGGTCCTTTCGATCTTTTAATAGCTTCTCAAGCGTT
ATCGAATAAACTTACTCTCGTAACAAACAATGAAAAAGAGTTTACTCGGATCAAAGATATTAAAATTGCAAACTGGCTTT
AA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216162 WP_000004570.1 NZ_CP083129:c4775590-4775303 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216162 NZ_CP112978:2481836-2482066 [Leptospira borgpetersenii]
ATGAATAAAGCTAAAATTTTTAAAAATGGGGATAGTCAAGCAATTCGTTTACCGAAAGACTATCGTTTCAAAGGCAAAGA
AGTCTATATACGAAGGGATGGAGATAATGTTATCTTAACACCAATAAACGATGCGGTTGATCGTTTTTGGAATACGGTTA
ATAGCTTTTCTGACGATCTTATACTTGATCGAAACCAACCAAACGAGTATGGCAAACGAAATAAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References