Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2782811..2783789 Replicon chromosome
Accession NZ_CP083129
Organism Bacillus thuringiensis strain NB-176

Toxin (Protein)


Gene name spoIISA Uniprot ID B7IX38
Locus tag K8Z23_RS14290 Protein ID WP_000624981.1
Coordinates 2783052..2783789 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID B7IX39
Locus tag K8Z23_RS14285 Protein ID WP_000237817.1
Coordinates 2782811..2782939 (-) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z23_RS14255 (K8Z23_14225) 2778328..2778579 + 252 WP_000821942.1 LuxR C-terminal-related transcriptional regulator -
K8Z23_RS14260 (K8Z23_14230) 2778720..2779325 - 606 WP_000517276.1 hypothetical protein -
K8Z23_RS14265 (K8Z23_14235) 2779351..2780160 - 810 WP_016077711.1 papain-like cysteine protease family protein -
K8Z23_RS14270 (K8Z23_14240) 2780473..2781942 - 1470 WP_000287540.1 beta-Ala-His dipeptidase -
K8Z23_RS14275 (K8Z23_14245) 2782154..2782543 + 390 WP_000712655.1 YxeA family protein -
K8Z23_RS14280 (K8Z23_14250) 2782562..2782738 - 177 WP_000852629.1 stage II sporulation protein SB -
K8Z23_RS14285 (K8Z23_14255) 2782811..2782939 - 129 WP_000237817.1 hypothetical protein Antitoxin
K8Z23_RS14290 (K8Z23_14260) 2783052..2783789 - 738 WP_000624981.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
K8Z23_RS14295 (K8Z23_14265) 2784028..2784765 - 738 WP_000594159.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
K8Z23_RS14300 (K8Z23_14270) 2784931..2785413 - 483 WP_000191905.1 MarR family winged helix-turn-helix transcriptional regulator -
K8Z23_RS14305 (K8Z23_14275) 2785561..2787204 + 1644 WP_016077712.1 alpha-keto acid decarboxylase family protein -
K8Z23_RS14310 (K8Z23_14280) 2787362..2788144 - 783 WP_016077713.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28335.09 Da        Isoelectric Point: 8.2998

>T216161 WP_000624981.1 NZ_CP083129:c2783789-2783052 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T216161 NZ_CP112978:480874-481197 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTGGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 4981.37 Da        Isoelectric Point: 3.9150

>AT216161 WP_000237817.1 NZ_CP083129:c2782939-2782811 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Download         Length: 129 bp

>AT216161 NZ_CP112978:480632-480880 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6D1SWF1

Antitoxin

Source ID Structure
AlphaFold DB B7IX39

References