Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4834319..4834961 Replicon chromosome
Accession NZ_CP083116
Organism Bacillus thuringiensis strain SA11

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z41_RS25000 Protein ID WP_000635965.1
Coordinates 4834319..4834669 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z41_RS25005 Protein ID WP_000004570.1
Coordinates 4834674..4834961 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z41_RS24985 (K8Z41_24940) 4831254..4831712 - 459 WP_000344239.1 SprT family protein -
K8Z41_RS24990 (K8Z41_24945) 4831908..4832024 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K8Z41_RS24995 (K8Z41_24950) 4832083..4834251 - 2169 WP_000426236.1 Tex family protein -
K8Z41_RS25000 (K8Z41_24955) 4834319..4834669 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z41_RS25005 (K8Z41_24960) 4834674..4834961 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z41_RS25010 (K8Z41_24965) 4835270..4836439 - 1170 WP_000390616.1 alanine racemase -
K8Z41_RS25015 (K8Z41_24970) 4836557..4837507 - 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
K8Z41_RS25020 (K8Z41_24975) 4837664..4838023 - 360 WP_000583417.1 holo-ACP synthase -
K8Z41_RS25025 (K8Z41_24980) 4838117..4838689 + 573 WP_000908522.1 rhomboid family intramembrane serine protease -
K8Z41_RS25030 (K8Z41_24985) 4838682..4839644 - 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216160 WP_000635965.1 NZ_CP083116:c4834669-4834319 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216160 NZ_CP112978:480632-480880 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216160 WP_000004570.1 NZ_CP083116:c4834961-4834674 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216160 NZ_CP112978:480874-481197 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTGGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References