Detailed information of TA system TA216159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2475078..2476056
• Replicon: chromosome
• Accession: NZ_CP083116
• Organism: Bacillus thuringiensis strain SA11

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: K8Z41_RS12590
• Protein ID: WP_000624977.1
• Coordinates: 2475319..2476056 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: K8Z41_RS12585
• Protein ID: WP_000237818.1
• Coordinates: 2475078..2475206 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z41_RS12555 (K8Z41_12535)	2470566..2470817	+	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-
K8Z41_RS12560 (K8Z41_12540)	2471057..2471659	-	603	WP_000517260.1	hypothetical protein	-
K8Z41_RS12565 (K8Z41_12545)	2471685..2472494	-	810	WP_000678509.1	papain-like cysteine protease family protein	-
K8Z41_RS12570 (K8Z41_12550)	2472740..2474209	-	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
K8Z41_RS12575 (K8Z41_12555)	2474421..2474810	+	390	WP_000713866.1	YxeA family protein	-
K8Z41_RS12580 (K8Z41_12560)	2474829..2475005	-	177	WP_000852629.1	stage II sporulation protein SB	-
K8Z41_RS12585 (K8Z41_12565)	2475078..2475206	-	129	WP_000237818.1	hypothetical protein	Antitoxin
K8Z41_RS12590 (K8Z41_12570)	2475319..2476056	-	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K8Z41_RS12595 (K8Z41_12575)	2476294..2477031	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
K8Z41_RS12600 (K8Z41_12580)	2477198..2477680	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
K8Z41_RS12605 (K8Z41_12585)	2477788..2479473	+	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
K8Z41_RS12610 (K8Z41_12590)	2479631..2480413	-	783	WP_000381800.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T216159 WP_000624977.1 NZ_CP083116:c2476056-2475319 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T216159 NZ_CP112976:540190-540531 [Leptospira kirschneri]
ATGATTCGTGGTGAGATCTGGTGGGTAGACTTAGGAATTCCATTCGGAAGTGAACCTGGGTTTAAGCGTCCAGTTTTGAT
TATTCAAGATGACTCTTTTAATCAAAGTAATATCAGTACTGTAGTATCGATTGCGATTACATCAAATTTAAATTTGTCTG
AAGCTCCTGGCAACGTTTTGATCGGTAAGAAAGAATCCAGCTTATCAAAAGATTCAGTTGTGAATGTCTCTCAAATTGTG
ACTTTAGATAAGGAAAGGTTTATTGAACGAGTGGGAAAGCTTAAATCAAGTAAGATCAATGAAGTTGAAGTAGGTCTCAA
ATTAGTTACAGGTTTAGATTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT216159 WP_000237818.1 NZ_CP083116:c2475206-2475078 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT216159 NZ_CP112976:539955-540203 [Leptospira kirschneri]
ATGAAAACTGCAATTTCGATTCCAGATGAATTATTCAAGACTGCTGAGAAAACGGCAAAAAAACTTGGAATACCTCGAAG
CCAACTCTTTGCAAAAGCATTAGAAGAATTTATTCAATCCCATAGTAAAGAATCTGTAACAGAGAAATTAAATAAAATTT
ATAACAGAAAATCCAAAGAACTTAAAAGTAATATCACAGATTTATCTGTAGAGTTATTGCGCAAGAGTTTAAAAAATGAT
TCGTGGTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5