Detailed information of TA system TA216155

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 61465..62207
• Replicon: plasmid pCH_183-e
• Accession: NZ_CP083106
• Organism: Bacillus thuringiensis strain ABTS-351

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: K8Z42_RS33820
• Protein ID: WP_016097319.1
• Coordinates: 61623..62207 (+)
• Length: 195 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	61465..61561 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z42_RS33800 (57015)	57015..58646	+	1632	WP_016097315.1	ArdC-like ssDNA-binding domain-containing protein	-
K8Z42_RS33805 (58708)	58708..59694	+	987	WP_016097316.1	hypothetical protein	-
K8Z42_RS33810 (59716)	59716..60267	+	552	WP_016097317.1	metal-dependent hydrolase	-
K8Z42_RS33815 (60347)	60347..61372	+	1026	WP_016097318.1	DUF3991 and TOPRIM domain-containing protein	-
- (61465)	61465..61561	+	97	NuclAT_0	-	Antitoxin
- (61465)	61465..61561	+	97	NuclAT_0	-	Antitoxin
- (61465)	61465..61561	+	97	NuclAT_0	-	Antitoxin
- (61465)	61465..61561	+	97	NuclAT_0	-	Antitoxin
K8Z42_RS33820 (61623)	61623..62207	+	585	WP_016097319.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
K8Z42_RS33825 (62275)	62275..63576	+	1302	WP_016097320.1	hypothetical protein	-
K8Z42_RS33830 (63683)	63683..64063	+	381	WP_016097321.1	hypothetical protein	-
K8Z42_RS33835 (64184)	64184..66325	+	2142	WP_016097322.1	type IA DNA topoisomerase	-
K8Z42_RS33840 (66355)	66355..66801	+	447	WP_016097323.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80699	80699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-169)

Sequences

Toxin

Protein

• Length: 195 a.a.
• Molecular weight: 22900.17 Da
• Isoelectric Point: 8.9694

>T216155 WP_016097319.1 NZ_CP083106:61623-62207 [Bacillus thuringiensis]
MANKDNPKFYTISTEYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDTDEKRIG
VLLVNNMIPVPEKECKEIDIAEKTAADPQYGNLMLKQYLFLKENMDRVTNKVEKVYKDVTVQGKPSHKQKFLKGVCCDFP
KLEEKCQEYKERDQAKERDKARRIAYMRQMGRER

Nucleotide

• Length: 585 bp

>T216155 NZ_CP112898:c1269541-1269251 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT216155 NZ_CP083106:61465-61561 [Bacillus thuringiensis]
GTAACCTTACTTTAGTAGGTGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Structures

Antitoxin