Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4832800..4833442 Replicon chromosome
Accession NZ_CP083101
Organism Bacillus thuringiensis strain ABTS-351

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z42_RS24980 Protein ID WP_000635965.1
Coordinates 4832800..4833150 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z42_RS24985 Protein ID WP_000004570.1
Coordinates 4833155..4833442 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z42_RS24965 (K8Z42_24950) 4829735..4830193 - 459 WP_000344239.1 SprT family protein -
K8Z42_RS24970 (K8Z42_24955) 4830389..4830505 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K8Z42_RS24975 (K8Z42_24960) 4830564..4832732 - 2169 WP_000426236.1 Tex family protein -
K8Z42_RS24980 (K8Z42_24965) 4832800..4833150 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z42_RS24985 (K8Z42_24970) 4833155..4833442 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z42_RS24990 (K8Z42_24975) 4833751..4834920 - 1170 WP_000390616.1 alanine racemase -
K8Z42_RS24995 (K8Z42_24980) 4835038..4835988 - 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
K8Z42_RS25000 (K8Z42_24985) 4836145..4836504 - 360 WP_000583417.1 holo-ACP synthase -
K8Z42_RS25005 (K8Z42_24990) 4836598..4837170 + 573 WP_000908522.1 rhomboid family intramembrane serine protease -
K8Z42_RS25010 (K8Z42_24995) 4837163..4838125 - 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216154 WP_000635965.1 NZ_CP083101:c4833150-4832800 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216154 NZ_CP112897:2259620-2259910 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216154 WP_000004570.1 NZ_CP083101:c4833442-4833155 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216154 NZ_CP112897:2259309-2259623 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References