Detailed information of TA system TA216153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2472798..2473776
• Replicon: chromosome
• Accession: NZ_CP083101
• Organism: Bacillus thuringiensis strain ABTS-351

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: K8Z42_RS12565
• Protein ID: WP_000624977.1
• Coordinates: 2473039..2473776 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: K8Z42_RS12560
• Protein ID: WP_000237818.1
• Coordinates: 2472798..2472926 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z42_RS12530 (K8Z42_12530)	2468286..2468537	+	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-
K8Z42_RS12535 (K8Z42_12535)	2468777..2469379	-	603	WP_000517260.1	hypothetical protein	-
K8Z42_RS12540 (K8Z42_12540)	2469405..2470214	-	810	WP_000678509.1	papain-like cysteine protease family protein	-
K8Z42_RS12545 (K8Z42_12545)	2470460..2471929	-	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
K8Z42_RS12550 (K8Z42_12550)	2472141..2472530	+	390	WP_000713866.1	YxeA family protein	-
K8Z42_RS12555 (K8Z42_12555)	2472549..2472725	-	177	WP_000852629.1	stage II sporulation protein SB	-
K8Z42_RS12560 (K8Z42_12560)	2472798..2472926	-	129	WP_000237818.1	hypothetical protein	Antitoxin
K8Z42_RS12565 (K8Z42_12565)	2473039..2473776	-	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K8Z42_RS12570 (K8Z42_12570)	2474014..2474751	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
K8Z42_RS12575 (K8Z42_12575)	2474918..2475400	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
K8Z42_RS12580 (K8Z42_12580)	2475508..2477193	+	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
K8Z42_RS12585 (K8Z42_12585)	2477351..2478133	-	783	WP_000381800.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T216153 WP_000624977.1 NZ_CP083101:c2473776-2473039 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T216153 NZ_CP112897:2259309-2259623 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT216153 WP_000237818.1 NZ_CP083101:c2472926-2472798 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT216153 NZ_CP112897:2259620-2259910 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5