Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 547333..547951 Replicon chromosome
Accession NC_008253
Organism Escherichia coli 536

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECP_RS02595 Protein ID WP_001291435.1
Coordinates 547333..547551 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECP_RS02600 Protein ID WP_000344800.1
Coordinates 547577..547951 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECP_RS02565 542619..543192 + 574 Protein_492 YbaY family lipoprotein -
ECP_RS02570 543223..543534 - 312 WP_000409908.1 MGMT family protein -
ECP_RS02575 543913..544266 + 354 WP_000878138.1 DUF1428 family protein -
ECP_RS02580 544308..545858 - 1551 WP_011579086.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECP_RS02585 546022..546492 - 471 WP_000136192.1 YlaC family protein -
ECP_RS02590 546608..547159 - 552 WP_000102541.1 maltose O-acetyltransferase -
ECP_RS02595 547333..547551 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECP_RS02600 547577..547951 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECP_RS02605 548496..551645 - 3150 WP_001132481.1 multidrug efflux RND transporter permease subunit -
ECP_RS02610 551668..552860 - 1193 Protein_501 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T21615 WP_001291435.1 NC_008253:c547551-547333 [Escherichia coli 536]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T21615 NC_008253:c547551-547333 [Escherichia coli 536]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT21615 WP_000344800.1 NC_008253:c547951-547577 [Escherichia coli 536]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT21615 NC_008253:c547951-547577 [Escherichia coli 536]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References