Detailed information of TA system TA216149

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 54577..55319
• Replicon: plasmid pP05_2-d
• Accession: NZ_CP083089
• Organism: Bacillus thuringiensis strain B401

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: K8Z39_RS33710
• Protein ID: WP_016097319.1
• Coordinates: 54735..55319 (+)
• Length: 195 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	54577..54673 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z39_RS33690 (50127)	50127..51758	+	1632	WP_016097315.1	ArdC-like ssDNA-binding domain-containing protein	-
K8Z39_RS33695 (51820)	51820..52806	+	987	WP_016097316.1	hypothetical protein	-
K8Z39_RS33700 (52828)	52828..53379	+	552	WP_016097317.1	metal-dependent hydrolase	-
K8Z39_RS33705 (53459)	53459..54484	+	1026	WP_016097318.1	DUF3991 and TOPRIM domain-containing protein	-
- (54577)	54577..54673	+	97	NuclAT_0	-	Antitoxin
- (54577)	54577..54673	+	97	NuclAT_0	-	Antitoxin
- (54577)	54577..54673	+	97	NuclAT_0	-	Antitoxin
- (54577)	54577..54673	+	97	NuclAT_0	-	Antitoxin
K8Z39_RS33710 (54735)	54735..55319	+	585	WP_016097319.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
K8Z39_RS33715 (55387)	55387..56688	+	1302	WP_016097320.1	hypothetical protein	-
K8Z39_RS33720 (56795)	56795..57175	+	381	WP_016097321.1	hypothetical protein	-
K8Z39_RS33725 (57296)	57296..59437	+	2142	WP_016097322.1	type IA DNA topoisomerase	-
K8Z39_RS33730 (59467)	59467..59913	+	447	WP_016097323.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73803	73803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-169)

Sequences

Toxin

Protein

• Length: 195 a.a.
• Molecular weight: 22900.17 Da
• Isoelectric Point: 8.9694

>T216149 WP_016097319.1 NZ_CP083089:54735-55319 [Bacillus thuringiensis]
MANKDNPKFYTISTEYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDTDEKRIG
VLLVNNMIPVPEKECKEIDIAEKTAADPQYGNLMLKQYLFLKENMDRVTNKVEKVYKDVTVQGKPSHKQKFLKGVCCDFP
KLEEKCQEYKERDQAKERDKARRIAYMRQMGRER

Nucleotide

• Length: 585 bp

>T216149 NZ_CP112895:2258969-2259283 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Antitoxin

Nucleotide

• Length: 97 bp

>AT216149 NZ_CP083089:54577-54673 [Bacillus thuringiensis]
GTAACCTTACTTTAGTAGGTGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Structures

Antitoxin