Detailed information of TA system TA216148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3584015..3584993
• Replicon: chromosome
• Accession: NZ_CP083085
• Organism: Bacillus thuringiensis strain B401

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: K8Z39_RS19075
• Protein ID: WP_000624977.1
• Coordinates: 3584256..3584993 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: K8Z39_RS19070
• Protein ID: WP_000237818.1
• Coordinates: 3584015..3584143 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8Z39_RS19040 (K8Z39_19000)	3579502..3579754	+	253	Protein_3730	LuxR C-terminal-related transcriptional regulator	-
K8Z39_RS19045 (K8Z39_19005)	3579994..3580596	-	603	WP_000517260.1	hypothetical protein	-
K8Z39_RS19050 (K8Z39_19010)	3580622..3581431	-	810	WP_000678509.1	papain-like cysteine protease family protein	-
K8Z39_RS19055 (K8Z39_19015)	3581677..3583146	-	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
K8Z39_RS19060 (K8Z39_19020)	3583358..3583747	+	390	WP_000713866.1	YxeA family protein	-
K8Z39_RS19065 (K8Z39_19025)	3583766..3583942	-	177	WP_000852629.1	stage II sporulation protein SB	-
K8Z39_RS19070 (K8Z39_19030)	3584015..3584143	-	129	WP_000237818.1	hypothetical protein	Antitoxin
K8Z39_RS19075 (K8Z39_19035)	3584256..3584993	-	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K8Z39_RS19080 (K8Z39_19040)	3585231..3585968	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
K8Z39_RS19085 (K8Z39_19045)	3586135..3586617	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
K8Z39_RS19090 (K8Z39_19050)	3586725..3588410	+	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
K8Z39_RS19095 (K8Z39_19055)	3588568..3589350	-	783	WP_000381800.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T216148 WP_000624977.1 NZ_CP083085:c3584993-3584256 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T216148 NZ_CP112894:1436135-1436425 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT216148 WP_000237818.1 NZ_CP083085:c3584143-3584015 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT216148 NZ_CP112894:1435824-1436138 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5