Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 35320..35962 Replicon chromosome
Accession NZ_CP083085
Organism Bacillus thuringiensis strain B401

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag K8Z39_RS00320 Protein ID WP_000635965.1
Coordinates 35320..35670 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K8Z39_RS00325 Protein ID WP_000004570.1
Coordinates 35675..35962 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8Z39_RS00305 (K8Z39_00305) 32255..32713 - 459 WP_000344239.1 SprT family protein -
K8Z39_RS00310 (K8Z39_00310) 32909..33025 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K8Z39_RS00315 (K8Z39_00315) 33084..35252 - 2169 WP_000426236.1 Tex family protein -
K8Z39_RS00320 (K8Z39_00320) 35320..35670 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8Z39_RS00325 (K8Z39_00325) 35675..35962 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K8Z39_RS00330 (K8Z39_00330) 36271..37440 - 1170 WP_000390616.1 alanine racemase -
K8Z39_RS00335 (K8Z39_00335) 37558..38508 - 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
K8Z39_RS00340 (K8Z39_00340) 38665..39024 - 360 WP_000583417.1 holo-ACP synthase -
K8Z39_RS00345 (K8Z39_00345) 39118..39690 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
K8Z39_RS00350 (K8Z39_00350) 39683..40645 - 963 WP_000961168.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T216147 WP_000635965.1 NZ_CP083085:c35670-35320 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T216147 NZ_CP112894:1435824-1436138 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT216147 WP_000004570.1 NZ_CP083085:c35962-35675 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT216147 NZ_CP112894:1436135-1436425 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References