Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30753..31396 Replicon plasmid p3-0113481141
Accession NZ_CP083078
Organism Klebsiella pneumoniae strain 0113481141

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F42_RS28605 Protein ID WP_001044770.1
Coordinates 30753..31169 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F42_RS28610 Protein ID WP_001261282.1
Coordinates 31166..31396 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F42_RS28585 (K9F42_28480) 27716..28060 + 345 Protein_29 WbuC family cupin fold metalloprotein -
K9F42_RS28590 (K9F42_28485) 28107..28982 - 876 WP_000239590.1 extended-spectrum class A beta-lactamase CTX-M-15 -
K9F42_RS28600 (K9F42_28495) 29990..30679 - 690 Protein_32 AAA family ATPase -
K9F42_RS28605 (K9F42_28500) 30753..31169 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F42_RS28610 (K9F42_28505) 31166..31396 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F42_RS28615 (K9F42_28510) 31353..31814 + 462 WP_072202616.1 hypothetical protein -
K9F42_RS28620 (K9F42_28515) 31958..32308 + 351 WP_000493378.1 hypothetical protein -
K9F42_RS28625 (K9F42_28520) 32359..33102 + 744 WP_000129823.1 hypothetical protein -
K9F42_RS28630 (K9F42_28525) 33099..33875 + 777 WP_000015958.1 site-specific integrase -
K9F42_RS28635 (K9F42_28530) 33933..34190 - 258 WP_000764642.1 hypothetical protein -
K9F42_RS28640 (K9F42_28535) 34319..34423 - 105 WP_032409716.1 hypothetical protein -
K9F42_RS28645 (K9F42_28540) 34958..35824 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ere(A) / ARR-3 / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / sul1 / qacE / cmlA1 - 1..62913 62913
- inside Integron - - 3..62912 62909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T216146 WP_001044770.1 NZ_CP083078:c31169-30753 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T216146 NZ_CP112893:2263138-2263428 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTTGCAAGAGCGAGAGGAATGACGGAAT
TAGCAGAAAAAACGGGGTTGGGAAGAGAAAGCCTCTATAAAACATTATCACACGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT216146 WP_001261282.1 NZ_CP083078:c31396-31166 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT216146 NZ_CP112893:2262827-2263141 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References