Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 45686..46211 Replicon plasmid p1-0113481141
Accession NZ_CP083076
Organism Klebsiella pneumoniae strain 0113481141

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag K9F42_RS27455 Protein ID WP_001568026.1
Coordinates 45906..46211 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag K9F42_RS27450 Protein ID WP_001568025.1
Coordinates 45686..45904 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F42_RS27425 (41012) 41012..41719 + 708 WP_198065698.1 hypothetical protein -
K9F42_RS27430 (41801) 41801..42724 + 924 WP_052433692.1 J domain-containing protein -
K9F42_RS27435 (42842) 42842..44215 - 1374 WP_042015169.1 hypothetical protein -
K9F42_RS27440 (44479) 44479..45099 - 621 WP_042015168.1 hypothetical protein -
K9F42_RS27445 (45259) 45259..45516 + 258 Protein_38 hypothetical protein -
K9F42_RS27450 (45686) 45686..45904 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K9F42_RS27455 (45906) 45906..46211 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
K9F42_RS27460 (46380) 46380..46775 + 396 WP_072158608.1 hypothetical protein -
K9F42_RS27465 (46802) 46802..47125 + 324 WP_022644730.1 hypothetical protein -
K9F42_RS27470 (47122) 47122..48138 + 1017 WP_020315256.1 hypothetical protein -
K9F42_RS27475 (48473) 48473..49741 - 1269 WP_019705993.1 ISL3-like element ISKpn25 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..133444 133444
- flank IS/Tn - - 48473..49741 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T216145 WP_001568026.1 NZ_CP083076:45906-46211 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T216145 NZ_CP112893:2262827-2263141 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT216145 WP_001568025.1 NZ_CP083076:45686-45904 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT216145 NZ_CP112893:2263138-2263428 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTTGCAAGAGCGAGAGGAATGACGGAAT
TAGCAGAAAAAACGGGGTTGGGAAGAGAAAGCCTCTATAAAACATTATCACACGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References