Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5444067..5444692 Replicon chromosome
Accession NZ_CP083075
Organism Klebsiella pneumoniae strain 0113481141

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F42_RS26825 Protein ID WP_002882817.1
Coordinates 5444067..5444450 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F42_RS26830 Protein ID WP_004150355.1
Coordinates 5444450..5444692 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F42_RS26810 (5441433) 5441433..5442335 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F42_RS26815 (5442332) 5442332..5442967 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F42_RS26820 (5442964) 5442964..5443893 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F42_RS26825 (5444067) 5444067..5444450 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F42_RS26830 (5444450) 5444450..5444692 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F42_RS26835 (5444897) 5444897..5445814 + 918 WP_021466676.1 alpha/beta hydrolase -
K9F42_RS26840 (5445828) 5445828..5446769 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F42_RS26845 (5446814) 5446814..5447251 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F42_RS26850 (5447248) 5447248..5448108 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F42_RS26855 (5448102) 5448102..5448701 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-112)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T216144 WP_002882817.1 NZ_CP083075:c5444450-5444067 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T216144 NZ_CP112893:59801-60091 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTTGCAAGAGCGAGAGGAATGACGGAAT
TAGCAGAAAAAACGGGGTTGGGAAGAGAAAGCCTCTATAAAACATTATCACACGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT216144 WP_004150355.1 NZ_CP083075:c5444692-5444450 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT216144 NZ_CP112893:59490-59804 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References