Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4850050..4850860 Replicon chromosome
Accession NZ_CP083075
Organism Klebsiella pneumoniae strain 0113481141

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K9F42_RS24030 Protein ID WP_002887280.1
Coordinates 4850050..4850583 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F42_RS24035 Protein ID WP_002887278.1
Coordinates 4850594..4850860 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F42_RS24025 (4848882) 4848882..4850003 + 1122 WP_009309849.1 cupin domain-containing protein -
K9F42_RS24030 (4850050) 4850050..4850583 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K9F42_RS24035 (4850594) 4850594..4850860 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F42_RS24040 (4850963) 4850963..4852396 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F42_RS24045 (4852386) 4852386..4853069 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
K9F42_RS24050 (4853242) 4853242..4854627 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
K9F42_RS24055 (4854645) 4854645..4854950 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T216141 WP_002887280.1 NZ_CP083075:c4850583-4850050 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T216141 NZ_CP112892:c1269576-1269286 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT216141 WP_002887278.1 NZ_CP083075:c4850860-4850594 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT216141 NZ_CP112892:c1269887-1269573 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References