Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 358381..359216 Replicon chromosome
Accession NC_008253
Organism Escherichia coli 536

Toxin (Protein)


Gene name yeeV Uniprot ID Q83W73
Locus tag ECP_RS01695 Protein ID WP_000854700.1
Coordinates 358839..359216 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag ECP_RS01690 Protein ID WP_001278232.1
Coordinates 358381..358749 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECP_RS01665 356041..356859 + 819 WP_001234709.1 DUF945 domain-containing protein -
ECP_RS01670 356951..357436 + 486 WP_000213723.1 antirestriction protein -
ECP_RS01675 357452..357928 + 477 WP_001186199.1 RadC family protein -
ECP_RS01680 357997..358218 + 222 WP_000692353.1 DUF987 domain-containing protein -
ECP_RS01690 358381..358749 + 369 WP_001278232.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
ECP_RS01695 358839..359216 + 378 WP_000854700.1 TA system toxin CbtA family protein Toxin
ECP_RS01700 359213..359701 + 489 WP_000761726.1 hypothetical protein -
ECP_RS01705 359713..359910 + 198 WP_000839247.1 DUF957 domain-containing protein -
ECP_RS01710 359995..360849 + 855 WP_024174313.1 DUF4942 domain-containing protein -
ECP_RS01715 360942..362261 - 1320 WP_000144686.1 site-specific integrase -
ECP_RS01720 362627..362965 + 339 WP_000772639.1 Arm DNA-binding domain-containing protein -
ECP_RS01725 363400..363894 - 495 WP_001059463.1 MarR family transcriptional regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - vat / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 332648..408194 75546


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14276.28 Da        Isoelectric Point: 7.8046

>T21614 WP_000854700.1 NC_008253:358839-359216 [Escherichia coli 536]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T21614 NC_008253:358839-359216 [Escherichia coli 536]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT21614 WP_001278232.1 NC_008253:358381-358749 [Escherichia coli 536]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT21614 NC_008253:358381-358749 [Escherichia coli 536]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A454A1I2


Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References