Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5236494..5237119 Replicon chromosome
Accession NZ_CP083070
Organism Klebsiella pneumoniae strain 1230_0791_KP

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F20_RS25640 Protein ID WP_002882817.1
Coordinates 5236494..5236877 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F20_RS25645 Protein ID WP_004150355.1
Coordinates 5236877..5237119 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F20_RS25625 (5233860) 5233860..5234762 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F20_RS25630 (5234759) 5234759..5235394 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F20_RS25635 (5235391) 5235391..5236320 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F20_RS25640 (5236494) 5236494..5236877 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F20_RS25645 (5236877) 5236877..5237119 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F20_RS25650 (5237324) 5237324..5238241 + 918 WP_021466676.1 alpha/beta hydrolase -
K9F20_RS25655 (5238255) 5238255..5239196 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F20_RS25660 (5239241) 5239241..5239678 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F20_RS25665 (5239675) 5239675..5240535 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F20_RS25670 (5240529) 5240529..5241128 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T216123 WP_002882817.1 NZ_CP083070:c5236877-5236494 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T216123 NZ_CP112880:c4824059-4823808 [Streptomyces sp. CA-278952]
ATGCGCACGATGACCTACACCGAGTCCCGCGCGAAGTACGCCGAGACCCTGAGCGCCGTCGTGGACGACCGCGAGGAGGT
CGTGGTCACGCGCGCCGGGCATGAGCCCGTGGTGATAGTGGCGCTCGATGAGTACGAGTCCCTCAAAGAGACCGCGTACC
AGCTGCGGAGCCCGGAGAACGCCCGCCGCCTGCTGGCCTCGATCGACCGCCTGGAGAACGGCGGCGGCACGGTGCGGGAG
CTCGCGGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT216123 WP_004150355.1 NZ_CP083070:c5237119-5236877 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT216123 NZ_CP112880:c4823811-4823551 [Streptomyces sp. CA-278952]
GTGAAGTTCGTGTGGGACCAGTCGTCCTGGGACGACTACGTTTGGTGGCAGAACCAGGATCGCAAGATCCTCAAGAGGAT
CAACACACTGCTCCAGGACATCGCCCGCAACGGCAACGAGGGAATGGGAAAGCCGGAGCCCCTGAAGCACGGCTTCCAGG
GCTACTGGTCCCGGCGCATCACCGACGAGCACCGGCTCATCTACACGGTCGCCGGCGACGAGGTCCGTATCGCCGCCTGC
CGCTACCACTACGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References