Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4635960..4636770 Replicon chromosome
Accession NZ_CP083070
Organism Klebsiella pneumoniae strain 1230_0791_KP

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K9F20_RS22800 Protein ID WP_002887280.1
Coordinates 4635960..4636493 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F20_RS22805 Protein ID WP_002887278.1
Coordinates 4636504..4636770 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F20_RS22795 (4634792) 4634792..4635913 + 1122 WP_009309849.1 cupin domain-containing protein -
K9F20_RS22800 (4635960) 4635960..4636493 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K9F20_RS22805 (4636504) 4636504..4636770 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F20_RS22810 (4636873) 4636873..4638306 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F20_RS22815 (4638296) 4638296..4638979 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
K9F20_RS22820 (4639152) 4639152..4640537 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
K9F20_RS22825 (4640555) 4640555..4640860 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T216120 WP_002887280.1 NZ_CP083070:c4636493-4635960 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T216120 NZ_CP112877:116643-117224 [Pseudanabaena galeata CCNP1313]
ATGACTAGCAAGTTTACAGTGATATACGATGCCTGTGTCCTTTATTCCAACTATCTTAGAGATATTCTCATTCAATTGGC
GATCGCCGATCTCTTTAGAGCCAAATGGACTAACTTAATTCATGACGAATGGATTCGCAATCTCATCGAAAATCGTCCTG
ATTTACCAAAGGAAAAGCTAAATCAAGTCAAAGATTTGATGAATAGTCAAGTTCGAGACAGTTTAGTTACTGATTTTGAA
CAGCTAATTCCATCCTTAACCTTACCCGATCCTAACGATCACCATATTCTGGCGGCAGCAATTGTGGCTGAAGCTGATGT
TATTGTCACCTTTAATCTAAAAGATTTTCCCGATCTGAATATCAGTCAGTATGGAATCACAGCAAAGCATCCAGATGATT
TTATTGCCGATTTGATTGGCTTAAATCCTTTCAAAGTTATGGCAGCAGTTGAGACTTGTCGGCAACGCTTAAAAAAGCAA
CCCAAAACCAGCAATGAATACTTGGAAATTCTGTTAAAGCAGGGGCTACCACTCTCTGTGTCAATGCTAAAAGAACTCCA
GAATAATCAAAATACAATGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT216120 WP_002887278.1 NZ_CP083070:c4636770-4636504 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT216120 NZ_CP112877:116195-116650 [Pseudanabaena galeata CCNP1313]
ATGGTTGCCCTAACACAGTCTCTACACATTCCTACCGAGGAAGAAACCGAAATTTCTAAAGAAAGCAGCCGTATTCTTGC
TTCGCATATTAGCGATGGTGTCTGTCATCTCAAAATCGTAGAAGCTGATGGCAGTGAAGAAACAGCCACAATCCCTGCGG
CAGCCTATCGCCTATTTGTAGACATTTTGACCCAAATGTCCCAAGGCAATGCCGTAACGATCATCCCCATCCATGCTGAA
CTCACTACCCAAGAAGCCGCAGATTTAATCAATGTCTCGCGTCCCTTCCTAATCAAACAACTCGAAGAAGGCATAATTCC
CTATCACAAAGTCGGCACGCATCGCCGAGTTCGCTTTACTGATTTAATGGAATATAAAACAAATATTGATGCCGCCAGAA
ACAAAGTTTTAGATGAGATTGTGGCGATATCTGAAGAGATAGGACTATATGACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References