Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3991789..3992408 Replicon chromosome
Accession NZ_CP083070
Organism Klebsiella pneumoniae strain 1230_0791_KP

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K9F20_RS19690 Protein ID WP_002892050.1
Coordinates 3992190..3992408 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K9F20_RS19685 Protein ID WP_002892066.1
Coordinates 3991789..3992163 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F20_RS19675 (3986941) 3986941..3988134 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K9F20_RS19680 (3988157) 3988157..3991303 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K9F20_RS19685 (3991789) 3991789..3992163 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K9F20_RS19690 (3992190) 3992190..3992408 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K9F20_RS19695 (3992571) 3992571..3993137 + 567 WP_002892030.1 maltose O-acetyltransferase -
K9F20_RS19700 (3993109) 3993109..3993249 - 141 WP_004147370.1 hypothetical protein -
K9F20_RS19705 (3993270) 3993270..3993740 + 471 WP_020802585.1 YlaC family protein -
K9F20_RS19710 (3993715) 3993715..3995166 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
K9F20_RS19715 (3995267) 3995267..3995965 + 699 WP_023287311.1 GNAT family protein -
K9F20_RS19720 (3995962) 3995962..3996102 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K9F20_RS19725 (3996102) 3996102..3996365 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216119 WP_002892050.1 NZ_CP083070:3992190-3992408 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T216119 NZ_CP112877:c85866-85501 [Pseudanabaena galeata CCNP1313]
ATGCTTAGTGAAAACATCCCAATTTCTATCAGGTTTGCTGATGAGTTTGAAGAGAATTTATATCGTCTATCCAAAAGATT
TCGTAATATTCGTAAAGATGTTGTGGGTGTGATTGAGCAAATTCAGGCTGGTAATGTTGTGGGCGATCGCATTGCTGGTC
TGGGTGAAAATTATATTGTGATTAAGGTCAGGGTAAAGAATAGCAATATCCAAAAGGGGAAAAGCGCGGGTTATCGTTTG
GTTTATCAGGTGGAGTCTCCGACTAATGTACTGCTGTTGACTATCTATTCAAAGAGCGATCGCGATGACATTAGCGCTGC
TGAGATTTTGAATATTTTGTCTTCTATTTCTGTGGATGATGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT216119 WP_002892066.1 NZ_CP083070:3991789-3992163 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT216119 NZ_CP112877:c86110-85859 [Pseudanabaena galeata CCNP1313]
ATGAGAGCAGTGGAAGCTACGGGGACAATTGATCGCGAAGGGCATTTGTTGCTGGATCAGCCTTTTGGTGAGGACATATC
TAGTCGTGTCCGTGTGATTGTGTTGTTTTCTGAACCAGTTCAAGAGTTGGAAGCTGATCCTGATGATACGCCTATTGAGG
AGGTGAAGGCGAGTTTGAAACGAGCTTTTCAGCAAGCTAAGGCAGGGCAAACTAGACCAATTTCTGAATTGTGGGACAGA
ATTGATGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References