Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1472807..1473543 Replicon chromosome
Accession NZ_CP083070
Organism Klebsiella pneumoniae strain 1230_0791_KP

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag K9F20_RS07275 Protein ID WP_032433360.1
Coordinates 1473061..1473543 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K9F20_RS07270 Protein ID WP_003026799.1
Coordinates 1472807..1473073 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F20_RS07245 (1468453) 1468453..1469592 + 1140 WP_032433368.1 mannitol dehydrogenase -
K9F20_RS07250 (1469621) 1469621..1470283 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
K9F20_RS07255 (1470267) 1470267..1471271 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
K9F20_RS07260 (1471289) 1471289..1471921 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
K9F20_RS07265 (1471931) 1471931..1472494 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
K9F20_RS07270 (1472807) 1472807..1473073 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K9F20_RS07275 (1473061) 1473061..1473543 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
K9F20_RS07280 (1473904) 1473904..1474503 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
K9F20_RS07285 (1474716) 1474716..1475660 - 945 WP_077254249.1 fimbrial protein -
K9F20_RS07290 (1475672) 1475672..1476250 - 579 WP_032432061.1 type 1 fimbrial protein -
K9F20_RS07295 (1476254) 1476254..1476994 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1439975..1484486 44511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T216109 WP_032433360.1 NZ_CP083070:1473061-1473543 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T216109 NZ_CP112874:c3475728-3475435 [Pseudanabaena galeata CCNP1313]
ATTGAAGTTCGCCAGACAGAAATATTCGTCAACTGGTTTGTCAAATTAAGAGACAAAAAAGCCAAAGCCCGCATACAAGC
CCGTATCGATCGCATGGAAATGGGAAACTTTGGTGATGTTGCTCCAGTTGGACAAGGAGTAAGCGAAATGCGAATATTTT
ACGGCACTGGCTATAGAGTGTATTTTGTGCAGAGAGGTTCAATCCTTGTGATACTTCTATGTGGCGGCGATAAAAGCACT
CAGACTTCTGACATAAATAAAGCTAAGGAACTAGTTAAACAATTGGAGGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT216109 WP_003026799.1 NZ_CP083070:1472807-1473073 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT216109 NZ_CP112874:c3475431-3475138 [Pseudanabaena galeata CCNP1313]
ATGGCTATCACCACTACTCGATGGAACTCAGCAGAACATCTAAAAACAGAAGAAGATATTCAATTATATTTGGAAGCTTG
TCTTGAAGAAGCAGGTGATGCTCCTAACGTGATCATCCATGCATTTAGTGTTATCGCTCGCGCCAAGAACATGACTCAGT
TAGCAAAAGAATCAGGCTTAACAAGAGAGGGGCTATACAAGGCATTATCCCCTGATGGTAATCCTAGTTTTGCGACAGTG
ACAAAAGTTGCTAAAGCGCTTGGATATAAGCTGACCGTCCAGCCAGTTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References