Detailed information of TA system TA216104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5405..6048
• Replicon: plasmid p3-1613015451
• Accession: NZ_CP083069
• Organism: Klebsiella pneumoniae strain 1613015451

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K9F39_RS27485
• Protein ID: WP_114690528.1
• Coordinates: 5632..6048 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: K9F39_RS27480
• Protein ID: WP_001261276.1
• Coordinates: 5405..5635 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F39_RS27455 (K9F39_27425)	1519..2145	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
K9F39_RS27460	2142..2444	+	303	WP_004197636.1	hypothetical protein	-
K9F39_RS27465 (K9F39_27435)	2900..3691	-	792	WP_032667567.1	site-specific integrase	-
K9F39_RS27470 (K9F39_27440)	3688..4431	-	744	WP_004098973.1	hypothetical protein	-
K9F39_RS27475 (K9F39_27445)	4482..4832	-	351	WP_000493378.1	hypothetical protein	-
K9F39_RS27480 (K9F39_27450)	5405..5635	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K9F39_RS27485 (K9F39_27455)	5632..6048	+	417	WP_114690528.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9F39_RS27490 (K9F39_27460)	6399..7367	-	969	WP_084832609.1	IS5-like element IS903B family transposase	-
K9F39_RS27495 (K9F39_27465)	7401..7538	-	138	Protein_9	sugar dehydrogenase	-
K9F39_RS27500 (K9F39_27470)	7604..8305	-	702	WP_008502228.1	DsbA family oxidoreductase	-
K9F39_RS27505 (K9F39_27475)	8371..9477	-	1107	WP_006687059.1	alkene reductase	-
K9F39_RS27510 (K9F39_27480)	9690..10019	+	330	WP_087833142.1	thioredoxin family protein	-
K9F39_RS27515 (K9F39_27485)	10049..10387	-	339	WP_000888080.1	carboxymuconolactone decarboxylase family protein	-
K9F39_RS27520 (K9F39_27490)	10392..10973	-	582	WP_008502230.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / blaDHA-1 / sul1 / qnrB4	-	1..48821	48821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15181.66 Da
• Isoelectric Point: 8.5404

>T216104 WP_114690528.1 NZ_CP083069:5632-6048 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T216104 NZ_CP112874:3102474-3102737 [Pseudanabaena galeata CCNP1313]
ATGGGCGCAGCTATTAGAACCCGCATCATTAAAATCGGTAACTCTCAAGGTATTCGTATTCCCAAGTTACTCCTTGAGCA
AAGTGGTATTCATGCAGAAGTGGAAATTGAAGTGCAGGGCAGCCATCTGACTGTGCGCCCATTTCCACAATTACGCATGG
GATGGAATCAAGCCTTTGCGACGATGGCAGAGCAAGGAGACGATATTTTACTAGATGATATTAGCCCAACTGATTGGGAG
AGAGTTGATTGGGCGTGGGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT216104 WP_001261276.1 NZ_CP083069:5405-5635 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT216104 NZ_CP112874:3102728-3103057 [Pseudanabaena galeata CCNP1313]
GTGGGATTAAATGTCAAACGCTTTGATGTTTTTCTGGTTAATCTCGATCCCACTATTGGCAACGAAATTCAAAAAACACG
CCCATGTGTTGTGATTTCCCCCGATGAGATGAATCGCTATATTTCGACGGTGATTATTGCGCCTATGACGACTAGAGGGC
GCGTATATCCTACCCGCGTCATTTGTCAGTTTCAAGAAAAAGCAGGGCAAATAGTTATCGATCAAATTCGCACAGTTGAT
AAAACAAGATTAGTAAAGCGGCTTGGGCAAATCAGTCAGGATGAGCAAAAACTAGTTCTGGGAATTTTGGCAGAAATGTT
TGCTGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A387K023