Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 57459..58195 Replicon plasmid p1-1613015451
Accession NZ_CP083067
Organism Klebsiella pneumoniae strain 1613015451

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K9F39_RS26450 Protein ID WP_003026803.1
Coordinates 57713..58195 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K9F39_RS26445 Protein ID WP_003026799.1
Coordinates 57459..57725 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F39_RS26410 (K9F39_26380) 52905..53084 - 180 Protein_50 NUDIX domain-containing protein -
K9F39_RS26415 (K9F39_26385) 53077..54615 - 1539 WP_087759328.1 IS66 family transposase -
K9F39_RS26420 (K9F39_26390) 54665..55012 - 348 WP_087759329.1 IS66 family insertion sequence element accessory protein TnpB -
K9F39_RS26425 (K9F39_26395) 55009..55386 - 378 WP_023280007.1 transposase -
K9F39_RS26430 (K9F39_26400) 55479..56062 - 584 Protein_54 IS3-like element ISKpn1 family transposase -
K9F39_RS26435 (K9F39_26405) 56138..56422 - 285 WP_004187019.1 type II toxin-antitoxin system RelE/ParE family toxin -
K9F39_RS26440 (K9F39_26410) 56412..56660 - 249 WP_004187025.1 plasmid stabilization protein -
K9F39_RS26445 (K9F39_26420) 57459..57725 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K9F39_RS26450 (K9F39_26425) 57713..58195 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K9F39_RS26455 (K9F39_26430) 58403..59749 + 1347 WP_077249638.1 ISNCY family transposase -
K9F39_RS26460 (K9F39_26435) 59798..60202 + 405 WP_231539283.1 helix-turn-helix domain-containing protein -
K9F39_RS26465 (K9F39_26440) 60393..61739 + 1347 WP_077264206.1 ISNCY family transposase -
K9F39_RS26470 (K9F39_26445) 61793..61984 - 192 WP_060589184.1 hypothetical protein -
K9F39_RS26475 (K9F39_26450) 62339..62596 - 258 WP_023292103.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..165099 165099
- inside IScluster/Tn - - 47816..61739 13923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T216103 WP_003026803.1 NZ_CP083067:57713-58195 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T216103 NZ_CP112874:c2086308-2085967 [Pseudanabaena galeata CCNP1313]
ATGATTATTTGGATTGATGCTCAGTTGCCTCCAGCTTTGGCAAATTGGATAAATAGTAATTTTGATGTTGAGGCTATGTC
TTTGAAGTATCTCTCACTACGAGATGCGAAGGATATCGAAATCTTTGAGGCGGCAAGATTTGCTAATGTGGTGATTATGA
CGAAGGATAGCGATTTTATTGATTTGGTCTGTCGATTGGGATCGCCTCCTCGAATTTTATGGCTGACTTGTGGCAATGTC
TCCAATCGTAATTTGCAGCGACTTCTCACTGCTACTTTGGCTGAGGCATTAAGTCAATTGGAACAAGGGGAAGTTATTGT
AGAAATTAGTAATAATTCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT216103 WP_003026799.1 NZ_CP083067:57459-57725 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT216103 NZ_CP112874:c2086538-2086305 [Pseudanabaena galeata CCNP1313]
ATGAACTTAAAACATAGTCTACTAAATCGTATCACCCAAATTGCTGGGCAATGTGGTGGTCGTCCTTGTATTCGTGGGAT
GCGAATTCGGGTGACGGATATTTTGGAAATGCTATCGGAAAATGTAAGCATTACTGAAATTCTAGAAGATTTTCCTGATT
TAGAGCTTGCTGATATTCAAGCTTGTTTAGTCTTTGCGGCGCGGCGTACTGATTTCCCTAGACTTACTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References