Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 57459..58195 | Replicon | plasmid p1-1613015451 |
| Accession | NZ_CP083067 | ||
| Organism | Klebsiella pneumoniae strain 1613015451 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | L7SZ15 |
| Locus tag | K9F39_RS26450 | Protein ID | WP_003026803.1 |
| Coordinates | 57713..58195 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | L7SZ29 |
| Locus tag | K9F39_RS26445 | Protein ID | WP_003026799.1 |
| Coordinates | 57459..57725 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K9F39_RS26410 (K9F39_26380) | 52905..53084 | - | 180 | Protein_50 | NUDIX domain-containing protein | - |
| K9F39_RS26415 (K9F39_26385) | 53077..54615 | - | 1539 | WP_087759328.1 | IS66 family transposase | - |
| K9F39_RS26420 (K9F39_26390) | 54665..55012 | - | 348 | WP_087759329.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| K9F39_RS26425 (K9F39_26395) | 55009..55386 | - | 378 | WP_023280007.1 | transposase | - |
| K9F39_RS26430 (K9F39_26400) | 55479..56062 | - | 584 | Protein_54 | IS3-like element ISKpn1 family transposase | - |
| K9F39_RS26435 (K9F39_26405) | 56138..56422 | - | 285 | WP_004187019.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| K9F39_RS26440 (K9F39_26410) | 56412..56660 | - | 249 | WP_004187025.1 | plasmid stabilization protein | - |
| K9F39_RS26445 (K9F39_26420) | 57459..57725 | + | 267 | WP_003026799.1 | DUF1778 domain-containing protein | Antitoxin |
| K9F39_RS26450 (K9F39_26425) | 57713..58195 | + | 483 | WP_003026803.1 | GNAT family N-acetyltransferase | Toxin |
| K9F39_RS26455 (K9F39_26430) | 58403..59749 | + | 1347 | WP_077249638.1 | ISNCY family transposase | - |
| K9F39_RS26460 (K9F39_26435) | 59798..60202 | + | 405 | WP_231539283.1 | helix-turn-helix domain-containing protein | - |
| K9F39_RS26465 (K9F39_26440) | 60393..61739 | + | 1347 | WP_077264206.1 | ISNCY family transposase | - |
| K9F39_RS26470 (K9F39_26445) | 61793..61984 | - | 192 | WP_060589184.1 | hypothetical protein | - |
| K9F39_RS26475 (K9F39_26450) | 62339..62596 | - | 258 | WP_023292103.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..165099 | 165099 | |
| - | inside | IScluster/Tn | - | - | 47816..61739 | 13923 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17353.97 Da Isoelectric Point: 9.5822
>T216103 WP_003026803.1 NZ_CP083067:57713-58195 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T216103 NZ_CP112874:c2086308-2085967 [Pseudanabaena galeata CCNP1313]
ATGATTATTTGGATTGATGCTCAGTTGCCTCCAGCTTTGGCAAATTGGATAAATAGTAATTTTGATGTTGAGGCTATGTC
TTTGAAGTATCTCTCACTACGAGATGCGAAGGATATCGAAATCTTTGAGGCGGCAAGATTTGCTAATGTGGTGATTATGA
CGAAGGATAGCGATTTTATTGATTTGGTCTGTCGATTGGGATCGCCTCCTCGAATTTTATGGCTGACTTGTGGCAATGTC
TCCAATCGTAATTTGCAGCGACTTCTCACTGCTACTTTGGCTGAGGCATTAAGTCAATTGGAACAAGGGGAAGTTATTGT
AGAAATTAGTAATAATTCGTGA
ATGATTATTTGGATTGATGCTCAGTTGCCTCCAGCTTTGGCAAATTGGATAAATAGTAATTTTGATGTTGAGGCTATGTC
TTTGAAGTATCTCTCACTACGAGATGCGAAGGATATCGAAATCTTTGAGGCGGCAAGATTTGCTAATGTGGTGATTATGA
CGAAGGATAGCGATTTTATTGATTTGGTCTGTCGATTGGGATCGCCTCCTCGAATTTTATGGCTGACTTGTGGCAATGTC
TCCAATCGTAATTTGCAGCGACTTCTCACTGCTACTTTGGCTGAGGCATTAAGTCAATTGGAACAAGGGGAAGTTATTGT
AGAAATTAGTAATAATTCGTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10147.59 Da Isoelectric Point: 4.5256
>AT216103 WP_003026799.1 NZ_CP083067:57459-57725 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
Download Length: 267 bp
>AT216103 NZ_CP112874:c2086538-2086305 [Pseudanabaena galeata CCNP1313]
ATGAACTTAAAACATAGTCTACTAAATCGTATCACCCAAATTGCTGGGCAATGTGGTGGTCGTCCTTGTATTCGTGGGAT
GCGAATTCGGGTGACGGATATTTTGGAAATGCTATCGGAAAATGTAAGCATTACTGAAATTCTAGAAGATTTTCCTGATT
TAGAGCTTGCTGATATTCAAGCTTGTTTAGTCTTTGCGGCGCGGCGTACTGATTTCCCTAGACTTACTGCATGA
ATGAACTTAAAACATAGTCTACTAAATCGTATCACCCAAATTGCTGGGCAATGTGGTGGTCGTCCTTGTATTCGTGGGAT
GCGAATTCGGGTGACGGATATTTTGGAAATGCTATCGGAAAATGTAAGCATTACTGAAATTCTAGAAGATTTTCCTGATT
TAGAGCTTGCTGATATTCAAGCTTGTTTAGTCTTTGCGGCGCGGCGTACTGATTTCCCTAGACTTACTGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J2GNW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Q8YL66 |