Detailed information of TA system TA216102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 56138..56660
• Replicon: plasmid p1-1613015451
• Accession: NZ_CP083067
• Organism: Klebsiella pneumoniae strain 1613015451

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: K9F39_RS26435
• Protein ID: WP_004187019.1
• Coordinates: 56138..56422 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: K9F39_RS26440
• Protein ID: WP_004187025.1
• Coordinates: 56412..56660 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F39_RS26405 (K9F39_26375)	51290..52497	+	1208	WP_087788032.1	IS3 family transposase	-
K9F39_RS26410 (K9F39_26380)	52905..53084	-	180	Protein_50	NUDIX domain-containing protein	-
K9F39_RS26415 (K9F39_26385)	53077..54615	-	1539	WP_087759328.1	IS66 family transposase	-
K9F39_RS26420 (K9F39_26390)	54665..55012	-	348	WP_087759329.1	IS66 family insertion sequence element accessory protein TnpB	-
K9F39_RS26425 (K9F39_26395)	55009..55386	-	378	WP_023280007.1	transposase	-
K9F39_RS26430 (K9F39_26400)	55479..56062	-	584	Protein_54	IS3-like element ISKpn1 family transposase	-
K9F39_RS26435 (K9F39_26405)	56138..56422	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9F39_RS26440 (K9F39_26410)	56412..56660	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
K9F39_RS26445 (K9F39_26420)	57459..57725	+	267	WP_003026799.1	DUF1778 domain-containing protein	-
K9F39_RS26450 (K9F39_26425)	57713..58195	+	483	WP_003026803.1	GNAT family N-acetyltransferase	-
K9F39_RS26455 (K9F39_26430)	58403..59749	+	1347	WP_077249638.1	ISNCY family transposase	-
K9F39_RS26460 (K9F39_26435)	59798..60202	+	405	WP_231539283.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..165099	165099
	-	inside	IScluster/Tn	-	-	47816..61739	13923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T216102 WP_004187019.1 NZ_CP083067:c56422-56138 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T216102 NZ_CP112874:c549790-549422 [Pseudanabaena galeata CCNP1313]
ATGCCTAATGGAACGCTAACTTATAAGCGAGGAGAAATATGGTGGGTCGATCTGAACCCAATTGTCGGCAGTGAGACTGG
CAAAGAACGTCCTTGCTTAATTTTGCAAAATGACATTGGCAATCAAAATGGATTAACCACCATAGTTGCGCCATTACTGC
CTAATAAAAAAACTTATCCATTCGTTGTGAATGTTATACCGACCCCGAAGAATGGACTAAGCGGCGATCGGCATATTAAT
TTGAGTCAAATGAGGGCTGTAGATGCTCGAAGAATCAAGAATAAACAGGGTGTATTAGAGGATATCTATTGGAAAGAAAT
TGCGAAGGCTGTATCTATTGAGCTTGGCTTCAATGACTTCTCTTCTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT216102 WP_004187025.1 NZ_CP083067:c56660-56412 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT216102 NZ_CP112874:c550007-549783 [Pseudanabaena galeata CCNP1313]
ATGAGTAAGAAAGTCAGCATTACCTTGGATGACGAAGTTCTCAGCTTTGTTGATCGATTAGCAAGCAATCGCAGCAGTTT
TATCAACGACATCCTCTGGAAAGAGAAGCAGAGGATTTTTATGAAAGAACTAGAAAATGCTTATAAAGAACAAGCAAACG
ATCCAATGTTTCAAGAAGAGATTTCTGTTTGGGACGTTACTGTAGAAGATGGTTTAAATGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4