Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 10956..11599 Replicon plasmid p1-1613015451
Accession NZ_CP083067
Organism Klebsiella pneumoniae strain 1613015451

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F39_RS26215 Protein ID WP_001044770.1
Coordinates 11183..11599 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F39_RS26210 Protein ID WP_001261282.1
Coordinates 10956..11186 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F39_RS26180 (K9F39_26150) 6963..7085 + 123 WP_072157889.1 small membrane protein -
K9F39_RS26185 (K9F39_26155) 7375..8010 + 636 WP_023292101.1 hypothetical protein -
K9F39_RS26190 (K9F39_26160) 8168..8632 + 465 WP_171888149.1 hypothetical protein -
K9F39_RS26195 (K9F39_26170) 9278..9535 - 258 WP_023292103.1 hypothetical protein -
K9F39_RS26200 (K9F39_26175) 9841..10173 - 333 Protein_8 integrase core domain-containing protein -
K9F39_RS26205 (K9F39_26180) 10772..10999 - 228 Protein_9 hypothetical protein -
K9F39_RS26210 (K9F39_26185) 10956..11186 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F39_RS26215 (K9F39_26190) 11183..11599 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F39_RS26220 (K9F39_26195) 11673..13235 + 1563 WP_060613526.1 AAA family ATPase -
K9F39_RS26225 (K9F39_26200) 13220..14242 + 1023 WP_000361404.1 helicase UvrD -
K9F39_RS26230 (K9F39_26205) 14786..15694 + 909 WP_230796727.1 HNH endonuclease -
K9F39_RS26235 (K9F39_26210) 15880..16230 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..165099 165099


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T216101 WP_001044770.1 NZ_CP083067:11183-11599 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T216101 NZ_CP112873:2680044-2680223 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT216101 WP_001261282.1 NZ_CP083067:10956-11186 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT216101 NZ_CP112873:c2680369-2680147 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References