Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55117..55642 Replicon plasmid p2-1613258639
Accession NZ_CP083057
Organism Klebsiella pneumoniae strain 1613258639

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag K9F40_RS27725 Protein ID WP_001159868.1
Coordinates 55337..55642 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag K9F40_RS27720 Protein ID WP_000813634.1
Coordinates 55117..55335 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F40_RS27690 (K9F40_27670) 50510..50926 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
K9F40_RS27695 (K9F40_27675) 50923..51153 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
K9F40_RS27700 (K9F40_27680) 51418..51918 + 501 WP_000528931.1 HEPN family nuclease -
K9F40_RS27705 (K9F40_27685) 51931..52704 + 774 WP_000905949.1 hypothetical protein -
K9F40_RS27710 (K9F40_27690) 52871..54004 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
K9F40_RS27715 (K9F40_27695) 54038..54526 - 489 WP_011254646.1 hypothetical protein -
K9F40_RS27720 (K9F40_27700) 55117..55335 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K9F40_RS27725 (K9F40_27705) 55337..55642 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
K9F40_RS27730 (K9F40_27710) 55643..56449 + 807 WP_000016982.1 site-specific integrase -
K9F40_RS27735 (K9F40_27715) 57223..57978 + 756 WP_000852146.1 replication initiation protein RepE -
K9F40_RS27740 (K9F40_27720) 58566..59732 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iutA / iucD / iucC / iucB / iucA 1..73277 73277


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T216066 WP_001159868.1 NZ_CP083057:55337-55642 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T216066 NZ_CP111140:c2273471-2272566 [Burkholderia pseudomallei]
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCATCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCGAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGGATCGCAGGCGAGCGGCTGGCGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCTGCAGGT
GATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGACGCCTTTTGCCGTCAAT
GCGACGGATCGCGAGGCACACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT216066 WP_000813634.1 NZ_CP083057:55117-55335 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT216066 NZ_CP111140:c2272569-2271715 [Burkholderia pseudomallei]
ATGAGCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCT
GCGGATCCTGGCCGAGTTCTCGCCGCGAGAGCCGGTGCTCGGCGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCGGACAAGGATCGCAACTATCGCCTG
GGCGTCGCGGTGCTGCGCCTCGGCTTCGAATACCTGAGCTCGCTCGAGCTGACCGATCTCGGCTTGCCGGTGATCGAAAG
CCTGCGCGATGCGACGGGCTTCACCGCGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAAA
ACGCGGGGAACGCGTTCGGCTCGGTGCGGGTGAACGTCGGCACGCGCCTGCCCGCGCATGCGACGACGCACGGCCACGTG
CTGATGGGCGACCTGTCGCTCGCGCAGTTGCGCGCGTTGTATCCGGAGCGGGCGCTCGAACGCGTGACGAAGGCGACGCC
CGAGACCGTCGACGCGCTCTTCGACGTGATCCGCGACGATGCGCGGCGCGGCTACGGCATCGGCAACTCGGCGTTCGAGC
GCGACATCTCGGTCGTCACGGCGCCCGTGCGCAACGAAGCGAGCCGGATCGTCGCGGTCGTCACGGTGACGGTGCCGCGG
CCGGAGATCGATGCGGCGCTCGTCGCCGAAGGGCTCGTCGAGAAGGTCGTGCGTGCGGCGGCCGAGCTGTCGCGGCGGCT
GAACTATCGGTCCGACGACGAAAACACCTATTTGAAGGCATTGGGATTGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References