Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 45647..46290 Replicon plasmid p2-1613258639
Accession NZ_CP083057
Organism Klebsiella pneumoniae strain 1613258639

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag K9F40_RS27675 Protein ID WP_001034044.1
Coordinates 45647..46063 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag K9F40_RS27680 Protein ID WP_001261286.1
Coordinates 46060..46290 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F40_RS27655 (K9F40_27635) 40779..41693 - 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -
K9F40_RS27660 (K9F40_27640) 42049..42746 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
K9F40_RS27665 (K9F40_27645) 43000..44022 - 1023 WP_000361402.1 helicase UvrD -
K9F40_RS27670 (K9F40_27650) 44007..45572 - 1566 WP_001128474.1 AAA family ATPase -
K9F40_RS27675 (K9F40_27655) 45647..46063 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F40_RS27680 (K9F40_27660) 46060..46290 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F40_RS27685 (K9F40_27665) 46671..50465 + 3795 WP_001144732.1 hypothetical protein -
K9F40_RS27690 (K9F40_27670) 50510..50926 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
K9F40_RS27695 (K9F40_27675) 50923..51153 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iutA / iucD / iucC / iucB / iucA 1..73277 73277


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T216064 WP_001034044.1 NZ_CP083057:c46063-45647 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T216064 NZ_CP111139:c3924128-3923832 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGACGAACTGGAGGATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT216064 WP_001261286.1 NZ_CP083057:c46290-46060 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT216064 NZ_CP111139:c3923829-3923473 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References