Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5294732..5295357 Replicon chromosome
Accession NZ_CP083055
Organism Klebsiella pneumoniae strain 1613258639

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F40_RS25900 Protein ID WP_002882817.1
Coordinates 5294732..5295115 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F40_RS25905 Protein ID WP_004150355.1
Coordinates 5295115..5295357 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F40_RS25885 (5292098) 5292098..5293000 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F40_RS25890 (5292997) 5292997..5293632 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F40_RS25895 (5293629) 5293629..5294558 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
K9F40_RS25900 (5294732) 5294732..5295115 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F40_RS25905 (5295115) 5295115..5295357 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F40_RS25910 (5295562) 5295562..5296479 + 918 WP_004181612.1 alpha/beta hydrolase -
K9F40_RS25915 (5296493) 5296493..5297434 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F40_RS25920 (5297479) 5297479..5297916 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
K9F40_RS25925 (5297913) 5297913..5298773 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F40_RS25930 (5298767) 5298767..5299366 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T216063 WP_002882817.1 NZ_CP083055:c5295115-5294732 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T216063 NZ_CP111139:c3923829-3923473 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT216063 WP_004150355.1 NZ_CP083055:c5295357-5295115 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT216063 NZ_CP111139:c3924128-3923832 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGACGAACTGGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References