Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4677385..4678195 Replicon chromosome
Accession NZ_CP083055
Organism Klebsiella pneumoniae strain 1613258639

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K9F40_RS23025 Protein ID WP_004178461.1
Coordinates 4677385..4677918 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F40_RS23030 Protein ID WP_002887278.1
Coordinates 4677929..4678195 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F40_RS23020 (4676216) 4676216..4677337 + 1122 WP_004214138.1 cupin domain-containing protein -
K9F40_RS23025 (4677385) 4677385..4677918 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K9F40_RS23030 (4677929) 4677929..4678195 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F40_RS23035 (4678298) 4678298..4679731 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F40_RS23040 (4679721) 4679721..4680404 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K9F40_RS23045 (4680576) 4680576..4681961 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
K9F40_RS23050 (4681979) 4681979..4682323 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T216061 WP_004178461.1 NZ_CP083055:c4677918-4677385 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T216061 NZ_CP111138:c2273801-2272896 [Burkholderia pseudomallei]
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCATCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCGAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGGATCGCAGGCGAGCGGCTGGCGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCTGCAGGT
GATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGACGCCTTTTGCCGTCAAT
GCGACGGATCGCGAGGCACACCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT216061 WP_002887278.1 NZ_CP083055:c4678195-4677929 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT216061 NZ_CP111138:c2272899-2272045 [Burkholderia pseudomallei]
ATGAGCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCT
GCGGATCCTGGCCGAGTTCTCGCCGCGAGAGCCGGTGCTCGGCGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCGGACAAGGATCGCAACTATCGCCTG
GGCGTCGCGGTGCTGCGCCTCGGCTTCGAATACCTGAGCTCGCTCGAGCTGACCGATCTCGGCTTGCCGGTGATCGAAAG
CCTGCGCGATGCGACGGGCTTCACCGCGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAAA
ACGCGGGGAACGCGTTCGGCTCGGTGCGGGTGAACGTCGGCACGCGCCTGCCCGCGCATGCGACGACGCACGGCCACGTG
CTGATGGGCGACCTGTCGCTCGCGCAGTTGCGCGCGTTGTATCCGGAGCGGGCGCTCGAACGCGTGACGAAGGCGACGCC
CGAGACCGTCGACGCGCTCTTCGACGTGATCCGCGACGATGCGCGGCGCGGCTACGGCATCGGCAACTCGGCGTTCGAGC
GCGACATCTCGGTCGTCACGGCGCCCGTGCGCAACGAAGCGAGCCGGATCGTCGCGGTCGTCACGGTGACGGTGCCGCGG
CCGGAGATCGATGCGGCGCTCGTCGCCGAAGGGCTCGTCGAGAAGGTCGTGCGTGCGGCGGCCGAGCTGTCGCGGCGGCT
GAACTATCGGTCCGACGACGAAAACACCTATTTGAAGGCATTGGGATTGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References