216047

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 61006..61757 Replicon plasmid p1-0213293972
Accession NZ_CP083052
Organism Klebsiella pneumoniae strain 0213293972

Toxin (Protein)


Gene name ataT Uniprot ID A0A486R6L7
Locus tag K9F37_RS26465 Protein ID WP_041165502.1
Coordinates 61275..61757 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A6S4YWG5
Locus tag K9F37_RS26460 Protein ID WP_012540170.1
Coordinates 61006..61284 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F37_RS26435 (K9F37_26360) 56534..57403 - 870 WP_001567390.1 DMT family transporter -
K9F37_RS26440 (K9F37_26365) 57644..58396 + 753 WP_004729622.1 alpha/beta hydrolase -
K9F37_RS26445 (K9F37_26370) 58447..58736 + 290 Protein_65 Tn3 family transposase -
K9F37_RS26450 (K9F37_26375) 58843..59157 - 315 WP_023288299.1 hypothetical protein -
K9F37_RS26455 (K9F37_26380) 60162..60698 - 537 WP_094986489.1 hypothetical protein -
K9F37_RS26460 (K9F37_26385) 61006..61284 + 279 WP_012540170.1 DUF1778 domain-containing protein Antitoxin
K9F37_RS26465 (K9F37_26390) 61275..61757 + 483 WP_041165502.1 GNAT family N-acetyltransferase Toxin
K9F37_RS26470 (K9F37_26395) 61795..62199 + 405 WP_048293664.1 DUF2251 domain-containing protein -
K9F37_RS26475 (K9F37_26400) 62519..63352 + 834 WP_012540133.1 GIY-YIG nuclease family protein -
K9F37_RS26480 (K9F37_26405) 63464..63613 + 150 WP_223884741.1 bacteriocin immunity protein -
K9F37_RS26485 (K9F37_26410) 63708..63944 - 237 WP_012540047.1 hypothetical protein -
K9F37_RS26490 (K9F37_26415) 64989..65717 + 729 WP_012540055.1 hypothetical protein -
K9F37_RS26495 (K9F37_26420) 66109..66261 - 153 WP_231545507.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..122698 122698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-144)

Antitoxin

Vibrio phage ICP1, Orf50

(6-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17697.61 Da        Isoelectric Point: 9.4948

>T216047 WP_041165502.1 NZ_CP083052:61275-61757 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIADFCCQDQVLSEWLKKKALKNHSTGLSRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTQHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Download         Length: 483 bp

>T216047 NZ_CP111115:c67410-67102 [Raoultella sp. DY2415]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCGG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10749.33 Da        Isoelectric Point: 4.6722

>AT216047 WP_012540170.1 NZ_CP083052:61006-61284 [Klebsiella pneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFNAFVEELDAPMTPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT216047 NZ_CP111115:c67649-67413 [Raoultella sp. DY2415]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486R6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A6S4YWG5

References