Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4610575..4611385 Replicon chromosome
Accession NZ_CP083051
Organism Klebsiella pneumoniae strain 0213293972

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag K9F37_RS22830 Protein ID WP_231544222.1
Coordinates 4610575..4611108 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F37_RS22835 Protein ID WP_002887278.1
Coordinates 4611119..4611385 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F37_RS22825 (4609406) 4609406..4610527 + 1122 WP_002887282.1 cupin domain-containing protein -
K9F37_RS22830 (4610575) 4610575..4611108 - 534 WP_231544222.1 type II toxin-antitoxin system toxin KacT Toxin
K9F37_RS22835 (4611119) 4611119..4611385 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F37_RS22840 (4611488) 4611488..4612921 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F37_RS22845 (4612911) 4612911..4613594 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K9F37_RS22850 (4613766) 4613766..4615151 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
K9F37_RS22855 (4615169) 4615169..4615513 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(95-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19827.74 Da        Isoelectric Point: 6.2369

>T216042 WP_231544222.1 NZ_CP083051:c4611108-4610575 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKMPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T216042 NZ_CP111114:1838878-1839090 [Pseudomonas koreensis]
GTGCAAAGCAGGCTATTGATCAAGGAGCTGGAGGAGGCAGGCTGGACGCTGGATCGAGTCATCGGCAGTCATCACATCTT
CACGCATCGTTACAACCCGTACACCATTCCCGTTCCTCACCCTAAAAAGGATTTGCCGCTGGGGACGGTCAAAAGCATCA
GGAGGCGTGCCGGGTTGTACCACCCGCCAGCCAGCTACAAGGGAGATCCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT216042 WP_002887278.1 NZ_CP083051:c4611385-4611119 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT216042 NZ_CP111114:1839090-1839494 [Pseudomonas koreensis]
ATGCAATATCCGATCTGCATCGAATGGGGTGACGACAACACCGCCATCGGTATTCAGATACCTGACATTCCCGGCGCCGT
CACGGCCGGGGACACTTTCGAGGACGCCTATAACGCAGCCGTTGAAGTCGCCCATCTCATGTTGCAGGAGATGGCGGCGG
ACGGGGAATCGATTCCGATGCCGACGTCGGCAGCGGCCCATCGCAATAATCCGGAGTTTGCCGACATGGGCTGGGGGATG
CTCGAGCTGGATATCTCGCCGTACATGGGCAAGACCGAGAAGGTCAACGTGACGTTGCCAGGCTATGTGATCCAGCGCAT
CGATCGCTATGTGCGTGAACACAATGTCAAAAGCCGCTCTTCGTTTCTGGCAGATGCGGCGATGGAGAAGCTGGTTCGGT
ATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References