Detailed information of TA system TA216042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4610575..4611385
• Replicon: chromosome
• Accession: NZ_CP083051
• Organism: Klebsiella pneumoniae strain 0213293972

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: K9F37_RS22830
• Protein ID: WP_231544222.1
• Coordinates: 4610575..4611108 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: K9F37_RS22835
• Protein ID: WP_002887278.1
• Coordinates: 4611119..4611385 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F37_RS22825 (4609406)	4609406..4610527	+	1122	WP_002887282.1	cupin domain-containing protein	-
K9F37_RS22830 (4610575)	4610575..4611108	-	534	WP_231544222.1	type II toxin-antitoxin system toxin KacT	Toxin
K9F37_RS22835 (4611119)	4611119..4611385	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
K9F37_RS22840 (4611488)	4611488..4612921	-	1434	WP_004192214.1	Cu(+)/Ag(+) sensor histidine kinase	-
K9F37_RS22845 (4612911)	4612911..4613594	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
K9F37_RS22850 (4613766)	4613766..4615151	+	1386	WP_004192218.1	efflux transporter outer membrane subunit	-
K9F37_RS22855 (4615169)	4615169..4615513	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (95-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19827.74 Da
• Isoelectric Point: 6.2369

>T216042 WP_231544222.1 NZ_CP083051:c4611108-4610575 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKMPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T216042 NZ_CP111114:1838878-1839090 [Pseudomonas koreensis]
GTGCAAAGCAGGCTATTGATCAAGGAGCTGGAGGAGGCAGGCTGGACGCTGGATCGAGTCATCGGCAGTCATCACATCTT
CACGCATCGTTACAACCCGTACACCATTCCCGTTCCTCACCCTAAAAAGGATTTGCCGCTGGGGACGGTCAAAAGCATCA
GGAGGCGTGCCGGGTTGTACCACCCGCCAGCCAGCTACAAGGGAGATCCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT216042 WP_002887278.1 NZ_CP083051:c4611385-4611119 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT216042 NZ_CP111114:1839090-1839494 [Pseudomonas koreensis]
ATGCAATATCCGATCTGCATCGAATGGGGTGACGACAACACCGCCATCGGTATTCAGATACCTGACATTCCCGGCGCCGT
CACGGCCGGGGACACTTTCGAGGACGCCTATAACGCAGCCGTTGAAGTCGCCCATCTCATGTTGCAGGAGATGGCGGCGG
ACGGGGAATCGATTCCGATGCCGACGTCGGCAGCGGCCCATCGCAATAATCCGGAGTTTGCCGACATGGGCTGGGGGATG
CTCGAGCTGGATATCTCGCCGTACATGGGCAAGACCGAGAAGGTCAACGTGACGTTGCCAGGCTATGTGATCCAGCGCAT
CGATCGCTATGTGCGTGAACACAATGTCAAAAGCCGCTCTTCGTTTCTGGCAGATGCGGCGATGGAGAAGCTGGTTCGGT
ATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLZ1