Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 59446..60182 Replicon plasmid p2-400195-1-18
Accession NZ_CP083049
Organism Klebsiella pneumoniae strain 400195-1-18

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K9F24_RS28045 Protein ID WP_003026803.1
Coordinates 59700..60182 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K9F24_RS28040 Protein ID WP_003026799.1
Coordinates 59446..59712 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F24_RS27995 (K9F24_27920) 55508..55870 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
K9F24_RS28000 (K9F24_27925) 55920..56270 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
K9F24_RS28005 (K9F24_27930) 56628..56897 + 270 WP_004152102.1 hypothetical protein -
K9F24_RS28010 (K9F24_27935) 56885..57460 + 576 WP_004152103.1 hypothetical protein -
K9F24_RS28015 (K9F24_27940) 57491..57985 + 495 WP_004152104.1 DNA-binding protein -
K9F24_RS28020 (K9F24_27945) 58029..58397 + 369 WP_004152105.1 hypothetical protein -
K9F24_RS28025 (K9F24_27950) 58431..58634 + 204 WP_004152106.1 HHA domain-containing protein -
K9F24_RS28030 (K9F24_27955) 58683..58940 + 258 WP_004152107.1 hypothetical protein -
K9F24_RS28035 (K9F24_27960) 59016..59270 + 255 WP_004152108.1 hypothetical protein -
K9F24_RS28040 (K9F24_27965) 59446..59712 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K9F24_RS28045 (K9F24_27970) 59700..60182 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K9F24_RS28050 (K9F24_27975) 60383..61786 + 1404 WP_001272054.1 ISNCY-like element ISKpn21 family transposase -
K9F24_RS28055 (K9F24_27980) 61815..62447 - 633 WP_001567369.1 hypothetical protein -
K9F24_RS28060 (K9F24_27985) 62677..64023 + 1347 WP_020314316.1 ISNCY family transposase -
K9F24_RS28930 64184..64315 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..216901 216901


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T216026 WP_003026803.1 NZ_CP083049:59700-60182 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T216026 NZ_CP111035:194817-195140 [Pseudomonas aeruginosa]
ATGGCGTTGCCGTCCGACGTGCGTCGCCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGC
AAAGGTGCTCAAGGGGTTCGGTGGTGCCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTAT
ACACGGTCAAGTTTGCGGAAGCGGTGTTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAG
GCCGACATGGACATCATCCGCGCTCGGCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCA
TTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT216026 WP_003026799.1 NZ_CP083049:59446-59712 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT216026 NZ_CP111035:195121-195456 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References