Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 34922..35832 Replicon plasmid p1-400195-1-18
Accession NZ_CP083048
Organism Klebsiella pneumoniae strain 400195-1-18

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag K9F24_RS26615 Protein ID WP_004181896.1
Coordinates 35362..35832 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag K9F24_RS26610 Protein ID WP_004181895.1
Coordinates 34922..35365 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F24_RS26595 (K9F24_26530) 31555..33042 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
K9F24_RS26600 (K9F24_26535) 33128..33550 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
K9F24_RS26605 (K9F24_26540) 33550..34821 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
K9F24_RS26610 (K9F24_26545) 34922..35365 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
K9F24_RS26615 (K9F24_26550) 35362..35832 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
K9F24_RS26620 (K9F24_26555) 35942..36202 - 261 WP_004026352.1 hypothetical protein -
K9F24_RS26625 (K9F24_26560) 36773..37954 - 1182 WP_004883380.1 IS481 family transposase -
K9F24_RS26630 (K9F24_26565) 38026..38184 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
K9F24_RS26635 (K9F24_26570) 38787..39761 + 975 WP_043907013.1 hypothetical protein -
K9F24_RS26640 (K9F24_26575) 39959..40336 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..254008 254008
- flank IS/Tn - - 36773..37954 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T216023 WP_004181896.1 NZ_CP083048:35362-35832 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T216023 NZ_CP111034:3572102-3572680 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT216023 WP_004181895.1 NZ_CP083048:34922-35365 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT216023 NZ_CP111034:3571459-3572013 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References