Detailed information of TA system TA216002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34922..35832
• Replicon: plasmid p1-400195-2-18
• Accession: NZ_CP083044
• Organism: Klebsiella pneumoniae strain 400195-2-18

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: K9F25_RS26615
• Protein ID: WP_004181896.1
• Coordinates: 35362..35832 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: K9F25_RS26610
• Protein ID: WP_004181895.1
• Coordinates: 34922..35365 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F25_RS26595 (K9F25_26530)	31555..33042	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
K9F25_RS26600 (K9F25_26535)	33128..33550	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
K9F25_RS26605 (K9F25_26540)	33550..34821	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
K9F25_RS26610 (K9F25_26545)	34922..35365	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
K9F25_RS26615 (K9F25_26550)	35362..35832	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
K9F25_RS26620 (K9F25_26555)	35942..36202	-	261	WP_004026352.1	hypothetical protein	-
K9F25_RS26625 (K9F25_26560)	36773..37954	-	1182	WP_004883380.1	IS481 family transposase	-
K9F25_RS26630 (K9F25_26565)	38026..38184	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
K9F25_RS26635 (K9F25_26570)	38787..39761	+	975	WP_043907013.1	hypothetical protein	-
K9F25_RS26640 (K9F25_26575)	39959..40336	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	-	1..254008	254008
	-	flank	IS/Tn	-	-	36773..37954	1181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T216002 WP_004181896.1 NZ_CP083044:35362-35832 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T216002 NZ_CP111029:4405831-4406142 [Salmonella sp. 2018103]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT216002 WP_004181895.1 NZ_CP083044:34922-35365 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT216002 NZ_CP111029:4406139-4406585 [Salmonella sp. 2018103]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9