Detailed information of TA system TA215986

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 839599..840256
• Replicon: chromosome
• Accession: NZ_CP083043
• Organism: Klebsiella pneumoniae strain 400195-2-18

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: K9F25_RS04160
• Protein ID: WP_004181233.1
• Coordinates: 839846..840256 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: K9F25_RS04155
• Protein ID: WP_002916312.1
• Coordinates: 839599..839865 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F25_RS04130 (834755)	834755..836188	-	1434	WP_004181234.1	6-phospho-beta-glucosidase	-
K9F25_RS04135 (836307)	836307..837035	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
K9F25_RS04140 (837085)	837085..837396	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
K9F25_RS04145 (837560)	837560..838219	+	660	WP_002916317.1	hemolysin III family protein	-
K9F25_RS04150 (838370)	838370..839353	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
K9F25_RS04155 (839599)	839599..839865	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
K9F25_RS04160 (839846)	839846..840256	+	411	WP_004181233.1	protein YgfX	Toxin
K9F25_RS04165 (840263)	840263..840784	-	522	WP_004144730.1	flavodoxin FldB	-
K9F25_RS04170 (840885)	840885..841781	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
K9F25_RS04175 (841804)	841804..842517	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K9F25_RS04180 (842523)	842523..844256	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T215986 WP_004181233.1 NZ_CP083043:839846-840256 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T215986 NZ_CP111008:c4116111-4115761 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT215986 WP_002916312.1 NZ_CP083043:839599-839865 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT215986 NZ_CP111008:c4116356-4116105 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41