Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5402936..5403561 Replicon chromosome
Accession NZ_CP083036
Organism Klebsiella pneumoniae strain 4906.28

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F35_RS26605 Protein ID WP_002882817.1
Coordinates 5402936..5403319 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F35_RS26610 Protein ID WP_004150355.1
Coordinates 5403319..5403561 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F35_RS26590 (5400302) 5400302..5401204 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F35_RS26595 (5401201) 5401201..5401836 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F35_RS26600 (5401833) 5401833..5402762 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F35_RS26605 (5402936) 5402936..5403319 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F35_RS26610 (5403319) 5403319..5403561 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F35_RS26615 (5403766) 5403766..5404683 + 918 WP_032418237.1 alpha/beta hydrolase -
K9F35_RS26620 (5404697) 5404697..5405638 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F35_RS26625 (5405683) 5405683..5406120 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F35_RS26630 (5406117) 5406117..5406977 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F35_RS26635 (5406971) 5406971..5407570 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215984 WP_002882817.1 NZ_CP083036:c5403319-5402936 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215984 NZ_CP111008:3886560-3886817 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215984 WP_004150355.1 NZ_CP083036:c5403561-5403319 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215984 NZ_CP111008:3886829-3887374 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References