Detailed information of TA system TA215965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 161900..162543
• Replicon: plasmid p1-5208.51
• Accession: NZ_CP083033
• Organism: Klebsiella pneumoniae strain 5208.51

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K9F36_RS26425
• Protein ID: WP_014386165.1
• Coordinates: 162127..162543 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: K9F36_RS26420
• Protein ID: WP_032408893.1
• Coordinates: 161900..162130 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F36_RS26395 (K9F36_26375)	156973..157956	-	984	WP_001471744.1	NAD(P)H-quinone oxidoreductase	-
K9F36_RS26400 (K9F36_26380)	157975..159123	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
K9F36_RS26405 (K9F36_26385)	159294..160451	-	1158	WP_032408895.1	acetylornithine deacetylase	-
K9F36_RS26410 (K9F36_26390)	160467..161141	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
K9F36_RS26415 (K9F36_26395)	161146..161580	-	435	WP_000103648.1	RidA family protein	-
K9F36_RS26420 (K9F36_26400)	161900..162130	+	231	WP_032408893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K9F36_RS26425 (K9F36_26405)	162127..162543	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
K9F36_RS26430 (K9F36_26410)	162866..163834	+	969	WP_074422984.1	IS5-like element IS903B family transposase	-
K9F36_RS26435 (K9F36_26415)	164014..164388	-	375	WP_032408891.1	hypothetical protein	-
K9F36_RS26440 (K9F36_26420)	164444..164770	-	327	WP_004152639.1	hypothetical protein	-
K9F36_RS26445 (K9F36_26425)	164767..165495	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
K9F36_RS26450 (K9F36_26430)	165492..165923	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..182759	182759
	-	flank	IS/Tn	-	-	162866..163834	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T215965 WP_014386165.1 NZ_CP083033:162127-162543 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T215965 NZ_CP111008:572196-572492 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT215965 WP_032408893.1 NZ_CP083033:161900-162130 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT215965 NZ_CP111008:572494-572889 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA