Detailed information of TA system TA215962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5139781..5140406
• Replicon: chromosome
• Accession: NZ_CP083032
• Organism: Klebsiella pneumoniae strain 5208.51

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K9F36_RS25175
• Protein ID: WP_040182550.1
• Coordinates: 5139781..5140164 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: K9F36_RS25180
• Protein ID: WP_004150355.1
• Coordinates: 5140164..5140406 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F36_RS25160 (5137147)	5137147..5138049	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
K9F36_RS25165 (5138046)	5138046..5138681	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
K9F36_RS25170 (5138678)	5138678..5139607	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
K9F36_RS25175 (5139781)	5139781..5140164	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9F36_RS25180 (5140164)	5140164..5140406	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
K9F36_RS25185 (5140611)	5140611..5141528	+	918	WP_023302328.1	alpha/beta hydrolase	-
K9F36_RS25190 (5141542)	5141542..5142483	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
K9F36_RS25195 (5142528)	5142528..5142965	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
K9F36_RS25200 (5142962)	5142962..5143822	-	861	WP_004146232.1	virulence factor BrkB family protein	-
K9F36_RS25205 (5143816)	5143816..5144415	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T215962 WP_040182550.1 NZ_CP083032:c5140164-5139781 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T215962 NZ_CP111005:159565-160035 [Escherichia coli]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT215962 WP_004150355.1 NZ_CP083032:c5140406-5140164 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT215962 NZ_CP111005:159125-159568 [Escherichia coli]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9