Detailed information of TA system TA215959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4577150..4577960
• Replicon: chromosome
• Accession: NZ_CP083032
• Organism: Klebsiella pneumoniae strain 5208.51

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: K9F36_RS22540
• Protein ID: WP_014908042.1
• Coordinates: 4577150..4577683 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: K9F36_RS22545
• Protein ID: WP_023343051.1
• Coordinates: 4577694..4577960 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F36_RS22535 (4575981)	4575981..4577102	+	1122	WP_012737335.1	cupin domain-containing protein	-
K9F36_RS22540 (4577150)	4577150..4577683	-	534	WP_014908042.1	type II toxin-antitoxin system toxin KacT	Toxin
K9F36_RS22545 (4577694)	4577694..4577960	-	267	WP_023343051.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
K9F36_RS22550 (4578063)	4578063..4579496	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
K9F36_RS22555 (4579486)	4579486..4580169	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
K9F36_RS22560 (4580342)	4580342..4581727	+	1386	WP_040182471.1	efflux transporter outer membrane subunit	-
K9F36_RS22565 (4581745)	4581745..4582089	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T215959 WP_014908042.1 NZ_CP083032:c4577683-4577150 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T215959 NZ_CP111004:c4212302-4212129 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT215959 WP_023343051.1 NZ_CP083032:c4577960-4577694 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT215959 NZ_CP111004:c4212626-4212330 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A332NV98