Detailed information of TA system TA215945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 76612..77255
• Replicon: plasmid p1-6604.68-OXA48
• Accession: NZ_CP083027
• Organism: Klebsiella pneumoniae strain 6604.68

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: K9F26_RS27090
• Protein ID: WP_001044770.1
• Coordinates: 76839..77255 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: K9F26_RS27085
• Protein ID: WP_001261282.1
• Coordinates: 76612..76842 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F26_RS27050 (K9F26_27015)	72184..73050	-	867	WP_004118283.1	replication initiation protein	-
K9F26_RS27055 (K9F26_27020)	73585..73689	+	105	WP_032409716.1	hypothetical protein	-
K9F26_RS27060 (K9F26_27025)	73818..74075	+	258	WP_000764642.1	hypothetical protein	-
K9F26_RS27065 (K9F26_27030)	74133..74909	-	777	WP_000015958.1	site-specific integrase	-
K9F26_RS27070 (K9F26_27035)	74906..75649	-	744	WP_000129823.1	hypothetical protein	-
K9F26_RS27075 (K9F26_27040)	75700..76050	-	351	WP_000493378.1	hypothetical protein	-
K9F26_RS27080 (K9F26_27045)	76194..76655	-	462	WP_072202616.1	hypothetical protein	-
K9F26_RS27085 (K9F26_27050)	76612..76842	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K9F26_RS27090 (K9F26_27055)	76839..77255	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9F26_RS27095 (K9F26_27060)	77329..78018	+	690	Protein_103	AAA family ATPase	-
K9F26_RS27100 (K9F26_27065)	78073..78770	+	698	WP_223174630.1	IS1 family transposase	-
K9F26_RS27105 (K9F26_27070)	78777..78977	+	201	Protein_105	transposase	-
K9F26_RS27110 (K9F26_27075)	79233..80108	+	876	WP_000239590.1	extended-spectrum class A beta-lactamase CTX-M-15	-
K9F26_RS27115 (K9F26_27080)	80155..80499	-	345	Protein_107	WbuC family cupin fold metalloprotein	-
K9F26_RS27120 (K9F26_27085)	80501..81901	+	1401	Protein_108	Tn3-like element Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(D) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaOXA-48 / blaCTX-M-15 / dfrA14	-	1..94913	94913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T215945 WP_001044770.1 NZ_CP083027:76839-77255 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T215945 NZ_CP111004:1819220-1819323 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT215945 WP_001261282.1 NZ_CP083027:76612-76842 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT215945 NZ_CP111004:c1819361-1819090 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3