Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5350163..5350788 Replicon chromosome
Accession NZ_CP083026
Organism Klebsiella pneumoniae strain 6604.68

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F26_RS26215 Protein ID WP_002882817.1
Coordinates 5350163..5350546 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F26_RS26220 Protein ID WP_004150355.1
Coordinates 5350546..5350788 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F26_RS26200 (5347529) 5347529..5348431 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F26_RS26205 (5348428) 5348428..5349063 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F26_RS26210 (5349060) 5349060..5349989 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F26_RS26215 (5350163) 5350163..5350546 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F26_RS26220 (5350546) 5350546..5350788 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F26_RS26225 (5350993) 5350993..5351910 + 918 WP_032418237.1 alpha/beta hydrolase -
K9F26_RS26230 (5351924) 5351924..5352865 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F26_RS26235 (5352910) 5352910..5353347 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F26_RS26240 (5353344) 5353344..5354204 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F26_RS26245 (5354198) 5354198..5354797 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215944 WP_002882817.1 NZ_CP083026:c5350546-5350163 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215944 NZ_CP111004:c1665634-1665260 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215944 WP_004150355.1 NZ_CP083026:c5350788-5350546 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215944 NZ_CP111004:c1666091-1665723 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References