Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 76602..77245 Replicon plasmid p1-6611.48-OXA48
Accession NZ_CP083022
Organism Klebsiella pneumoniae strain 6611.48

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F27_RS27100 Protein ID WP_001044770.1
Coordinates 76829..77245 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F27_RS27095 Protein ID WP_001261282.1
Coordinates 76602..76832 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F27_RS27060 (K9F27_27025) 72174..73040 - 867 WP_004118283.1 replication initiation protein -
K9F27_RS27065 (K9F27_27030) 73575..73679 + 105 WP_032409716.1 hypothetical protein -
K9F27_RS27070 (K9F27_27035) 73808..74065 + 258 WP_000764642.1 hypothetical protein -
K9F27_RS27075 (K9F27_27040) 74123..74899 - 777 WP_000015958.1 site-specific integrase -
K9F27_RS27080 (K9F27_27045) 74896..75639 - 744 WP_000129823.1 hypothetical protein -
K9F27_RS27085 (K9F27_27050) 75690..76040 - 351 WP_000493378.1 hypothetical protein -
K9F27_RS27090 (K9F27_27055) 76184..76645 - 462 WP_072202616.1 hypothetical protein -
K9F27_RS27095 (K9F27_27060) 76602..76832 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F27_RS27100 (K9F27_27065) 76829..77245 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F27_RS27105 (K9F27_27070) 77319..78008 + 690 Protein_103 AAA family ATPase -
K9F27_RS27110 (K9F27_27075) 78063..78760 + 698 WP_223174630.1 IS1 family transposase -
K9F27_RS27115 (K9F27_27080) 78767..78967 + 201 Protein_105 transposase -
K9F27_RS27120 (K9F27_27085) 79223..80098 + 876 WP_000239590.1 extended-spectrum class A beta-lactamase CTX-M-15 -
K9F27_RS27125 (K9F27_27090) 80145..80489 - 345 Protein_107 WbuC family cupin fold metalloprotein -
K9F27_RS27130 (K9F27_27095) 80491..81891 + 1401 Protein_108 Tn3-like element Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(D) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaOXA-48 / blaCTX-M-15 / dfrA14 - 1..94932 94932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T215927 WP_001044770.1 NZ_CP083022:76829-77245 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215927 NZ_CP110999:3886967-3887224 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT215927 WP_001261282.1 NZ_CP083022:76602-76832 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215927 NZ_CP110999:3887236-3887781 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References