Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4155347..4155966 Replicon chromosome
Accession NZ_CP083021
Organism Klebsiella pneumoniae strain 6611.48

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K9F27_RS20510 Protein ID WP_002892050.1
Coordinates 4155748..4155966 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K9F27_RS20505 Protein ID WP_002892066.1
Coordinates 4155347..4155721 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F27_RS20495 (4150499) 4150499..4151692 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K9F27_RS20500 (4151715) 4151715..4154861 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K9F27_RS20505 (4155347) 4155347..4155721 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K9F27_RS20510 (4155748) 4155748..4155966 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K9F27_RS20515 (4156125) 4156125..4156691 + 567 WP_002892030.1 maltose O-acetyltransferase -
K9F27_RS20520 (4156663) 4156663..4156803 - 141 WP_004147370.1 hypothetical protein -
K9F27_RS20525 (4156824) 4156824..4157294 + 471 WP_002892026.1 YlaC family protein -
K9F27_RS20530 (4157269) 4157269..4158720 - 1452 WP_032419337.1 PLP-dependent aminotransferase family protein -
K9F27_RS20535 (4158821) 4158821..4159519 + 699 WP_002892021.1 GNAT family protein -
K9F27_RS20540 (4159516) 4159516..4159656 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K9F27_RS20545 (4159656) 4159656..4159919 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215923 WP_002892050.1 NZ_CP083021:4155748-4155966 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215923 NZ_CP110999:3312280-3312498 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215923 WP_002892066.1 NZ_CP083021:4155347-4155721 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215923 NZ_CP110999:3311880-3312254 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References