Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 65204..65847 Replicon plasmid p1-6709.15-I-OXA48
Accession NZ_CP083017
Organism Klebsiella pneumoniae strain 6709.15-I

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F34_RS26945 Protein ID WP_001044770.1
Coordinates 65204..65620 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F34_RS26950 Protein ID WP_001261282.1
Coordinates 65617..65847 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F34_RS26915 (K9F34_26875) 60558..61958 - 1401 Protein_86 Tn3-like element Tn3 family transposase -
K9F34_RS26920 (K9F34_26880) 61960..62304 + 345 Protein_87 WbuC family cupin fold metalloprotein -
K9F34_RS26925 (K9F34_26885) 62351..63226 - 876 WP_000239590.1 extended-spectrum class A beta-lactamase CTX-M-15 -
K9F34_RS26930 (K9F34_26890) 63482..63682 - 201 Protein_89 transposase -
K9F34_RS26940 (K9F34_26900) 64441..65130 - 690 Protein_91 AAA family ATPase -
K9F34_RS26945 (K9F34_26905) 65204..65620 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F34_RS26950 (K9F34_26910) 65617..65847 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F34_RS26955 (K9F34_26915) 65804..66265 + 462 WP_072202616.1 hypothetical protein -
K9F34_RS26960 (K9F34_26920) 66409..66759 + 351 WP_000493378.1 hypothetical protein -
K9F34_RS26965 (K9F34_26925) 66810..67553 + 744 WP_000129823.1 hypothetical protein -
K9F34_RS26970 (K9F34_26930) 67550..68326 + 777 WP_000015958.1 site-specific integrase -
K9F34_RS26975 (K9F34_26935) 68384..68641 - 258 WP_000764642.1 hypothetical protein -
K9F34_RS26980 (K9F34_26940) 68770..68874 - 105 WP_032409716.1 hypothetical protein -
K9F34_RS26985 (K9F34_26945) 69409..70275 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(D) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaOXA-48 / dfrA14 / blaCTX-M-15 - 1..94997 94997


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T215909 WP_001044770.1 NZ_CP083017:c65620-65204 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215909 NZ_CP110999:699917-700324 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT215909 WP_001261282.1 NZ_CP083017:c65847-65617 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215909 NZ_CP110999:699670-699936 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References