Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61225..61868 Replicon plasmid p1-6710.71
Accession NZ_CP083012
Organism Klebsiella pneumoniae strain 6710.71

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F28_RS27280 Protein ID WP_001044770.1
Coordinates 61452..61868 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F28_RS27275 Protein ID WP_001261282.1
Coordinates 61225..61455 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F28_RS27240 (K9F28_27195) 56797..57663 - 867 WP_004118283.1 replication initiation protein -
K9F28_RS27245 (K9F28_27200) 58198..58302 + 105 WP_032409716.1 hypothetical protein -
K9F28_RS27250 (K9F28_27205) 58431..58688 + 258 WP_000764642.1 hypothetical protein -
K9F28_RS27255 (K9F28_27210) 58746..59522 - 777 WP_000015958.1 site-specific integrase -
K9F28_RS27260 (K9F28_27215) 59519..60262 - 744 WP_000129823.1 hypothetical protein -
K9F28_RS27265 (K9F28_27220) 60313..60663 - 351 WP_000493378.1 hypothetical protein -
K9F28_RS27270 (K9F28_27225) 60807..61268 - 462 WP_072202616.1 hypothetical protein -
K9F28_RS27275 (K9F28_27230) 61225..61455 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F28_RS27280 (K9F28_27235) 61452..61868 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F28_RS27285 (K9F28_27240) 61942..62631 + 690 Protein_81 AAA family ATPase -
K9F28_RS27290 (K9F28_27245) 62686..63383 + 698 WP_223174630.1 IS1 family transposase -
K9F28_RS27295 (K9F28_27250) 63399..64556 - 1158 Protein_83 IS1380-like element ISEcp1 family transposase -
K9F28_RS27300 (K9F28_27255) 64750..64956 - 207 Protein_84 DUF4158 domain-containing protein -
K9F28_RS27305 (K9F28_27260) 65120..65677 + 558 WP_001235713.1 recombinase family protein -
K9F28_RS27310 (K9F28_27265) 65860..66720 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(D) / mph(A) / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / dfrA14 / aadA2 / cmlA1 / sul3 / floR / aac(3)-IIa - 1..91520 91520


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T215891 WP_001044770.1 NZ_CP083012:61452-61868 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215891 NZ_CP110996:2868447-2868758 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT215891 WP_001261282.1 NZ_CP083012:61225-61455 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215891 NZ_CP110996:2868759-2869049 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References