Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5385155..5385780 Replicon chromosome
Accession NZ_CP083011
Organism Klebsiella pneumoniae strain 6710.71

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F28_RS26515 Protein ID WP_002882817.1
Coordinates 5385155..5385538 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F28_RS26520 Protein ID WP_004150355.1
Coordinates 5385538..5385780 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F28_RS26500 (5382521) 5382521..5383423 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F28_RS26505 (5383420) 5383420..5384055 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F28_RS26510 (5384052) 5384052..5384981 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F28_RS26515 (5385155) 5385155..5385538 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F28_RS26520 (5385538) 5385538..5385780 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F28_RS26525 (5385985) 5385985..5386902 + 918 WP_032418237.1 alpha/beta hydrolase -
K9F28_RS26530 (5386916) 5386916..5387857 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F28_RS26535 (5387902) 5387902..5388339 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F28_RS26540 (5388336) 5388336..5389196 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F28_RS26545 (5389190) 5389190..5389789 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215890 WP_002882817.1 NZ_CP083011:c5385538-5385155 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215890 NZ_CP110996:2026837-2027301 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215890 WP_004150355.1 NZ_CP083011:c5385780-5385538 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215890 NZ_CP110996:2026502-2026837 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References